Absorption, distribution and elimination of fumonisin B1 metabolites in weaned piglets

J. Fodor, K. Balogh, M. Weber, M. Mézes, L. Kametler, R. Pósa, R. Mamet, J. Bauer, P. Horn, F. Kovács, M. Kovács

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The absorption, distribution and elimination of fumonisin B1 (and B2) after oral administration of Fusarium verticillioides (MRC 826) fungal culture, mixed into the experimental feed for 10 days, was studied in weaned barrows. In order to determine the absorption of FB1 from the feed marked by chromium oxide, a special T-cannula was implanted into the distal part of pigs’ ileum. During the feeding of toxin-containing diet (45 mg FB1 kg−1) and until the tenth day after the end of treatment, the total quantity of urine and faeces was collected and their toxin content analysed. At the end of the trial, samples of lung, liver, kidney, brain, muscle, and fat were also collected and their fumonisin content analysed by LC-MS. The fumonisins appeared to decrease the reduced glutathione content in blood plasma and red blood cell haemolysate, possibly associated with in vivo lipid peroxidation. From a data set of 80 individual data and the concentration and rate of C r and fumonisins (FB1, partially hydrolysed FB1 and aminopentol) in the chymus, it could be established that the accumulative absorption of fumonisin B1 was 3.9% ± 0.7%. In the chymus, the FB1 conversions into aminopentol and partially hydrolysed FB1 were 1.0 and 3.9%, respectively. The degree of metabolism in faeces was variable, although the main product was the partially hydrolysed form, with very small amounts of the aminopentol moiety being recovered. In the investigated tissues the FB1 conversion to aminopentol and partially hydrolysed FB1 was 30 and 20%, respectively.

Original languageEnglish
Pages (from-to)88-96
Number of pages9
JournalFood Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment
Volume25
Issue number1
DOIs
Publication statusPublished - Jan 1 2008

Keywords

  • Absorption
  • Biotransformation
  • Fumonisin
  • Lipid peroxidation
  • Metabolites

ASJC Scopus subject areas

  • Food Science
  • Chemistry(all)
  • Toxicology
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Fingerprint Dive into the research topics of 'Absorption, distribution and elimination of fumonisin B<sub>1</sub> metabolites in weaned piglets'. Together they form a unique fingerprint.

  • Cite this