Absorption and fluorescence spectroscopic study on complexation of oxazine 1 dye by calix[8]arenesulfonate

M. Kubinyi, T. Vidóczy, Olívia Varga, Kornél Nagy, I. Bitter

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

It has been established by combined absorption and fluorescence measurements that the cationic dye Oxazine 1 (OX) and the polyvalent anionic host calix[8]arenesuUbnate (SCA8) form two complexes in simultaneous reactions: OX + SCA8 ↔ OX·SCA8 (1), and OX·SCA8 + OX ↔ OX 2·SCA8 (2). The equilibrium constants for the two reactions, as functions of the ionic strength (I), and the absorption and fluorescence spectra of the two complex species have been determined by a least-squares fitting method from the experimental data. The variations of the binding constants with the ionic strength could be described on the basis of Debye-Hackd theory. The equilibrium constants are large; their values extrapolated to I = 0 are K(1) = 5.5 × 106 M-1 and K(2) = 4.4 × 105 M-1. The fluorescence of OX undergoes a strong static quenching upon complexation. These results indicate that the complexes are held together by strong electrostatic forces. The addition of non-fluorescent tetramethylammonium chloride to OX-SCA8 mixtures results in a dramatic fluorescent enhancement, which demonstrates the potential applicability of this supramolecular system In fluorescence asjays.

Original languageEnglish
Pages (from-to)134-139
Number of pages6
JournalApplied Spectroscopy
Volume59
Issue number1
DOIs
Publication statusPublished - Jan 2005

Fingerprint

Complexation
Coloring Agents
Dyes
dyes
Fluorescence
fluorescence
Equilibrium constants
Ionic strength
Electrostatic force
Quenching
quenching
chlorides
electrostatics
absorption spectra
oxazine 1
calix(8)arenesulfonate
augmentation

Keywords

  • Absorption
  • Calixarenesulfonates
  • Dye probes
  • Fluorescence
  • Partial least squares
  • PLS
  • Supramolecular complex

ASJC Scopus subject areas

  • Spectroscopy
  • Instrumentation

Cite this

Absorption and fluorescence spectroscopic study on complexation of oxazine 1 dye by calix[8]arenesulfonate. / Kubinyi, M.; Vidóczy, T.; Varga, Olívia; Nagy, Kornél; Bitter, I.

In: Applied Spectroscopy, Vol. 59, No. 1, 01.2005, p. 134-139.

Research output: Contribution to journalArticle

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