Absence of host-site influence on angiogenesis, blood flow, and permeability in transplanted RG-2 gliomas

P. Molnár, I. Fekete, Kurt E. Schlageter, Gregory D. Lapin, Dennis R. Groothuis

Research output: Contribution to journalArticle

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Abstract

The host site is believed to regulate tumor angiogenesis, which could result in site-dependent drug delivery parameters, greatly affecting experimental tumor research. In RG-2 rat gliomas we measured cellular proliferation; cell cycle time was the same for RG-2 cells in brain and s.c. tumors (25 h), and was the same for endothelial cells in these tumors (46 h). We measured the transcapillary transfer constant (K) of α- aminoisobutyric acid and blood flow (F) with iodoantipyrine in RG-2 gliomas transplanted into brain, liver, kidney, muscle, s.c. tissue, and into the abdominal cavity. Data was evaluated by quantitative autoradiography and direct tissue sampling. The variation of F (12.6-84.0 ml/g/min) and K (26.1-49.2 μl/g/min) in RG-2 tumors in the different host sites was less than in surrounding tumor-free tissue (F = 20-1500 ml/g/min and K = 1.6-700 μl/g/min). In contrast to other models, RG-2 does not result in tumors with host site-dependent behavior. The RG-2 tumor cells appear to participate in, if not dominate, the angiogenesis process regardless of the host site. Values of F and K were more dependent on tumor topography (center versus periphery) and local histological features (necrosis versus viable tumor) than host site. We believe that the methods used for data acquisition may introduce as much variability in Results as the tumors themselves and that to better understand how tumor angiogenesis affects the vascular phenotype, comparative studies are needed to validate the results obtained with newer methodologies.

Original languageEnglish
Pages (from-to)1085-1091
Number of pages7
JournalDrug Metabolism and Disposition
Volume27
Issue number9
Publication statusPublished - 1999

Fingerprint

Glioma
Tumors
Permeability
Blood
Neoplasms
Tissue
Brain
Aminoisobutyric Acids
Cells
Abdominal Cavity
Endothelial cells
Autoradiography
Brain Neoplasms
Drug delivery
Blood Vessels
Liver
Topography
Cell Cycle
Muscle
Necrosis

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Absence of host-site influence on angiogenesis, blood flow, and permeability in transplanted RG-2 gliomas. / Molnár, P.; Fekete, I.; Schlageter, Kurt E.; Lapin, Gregory D.; Groothuis, Dennis R.

In: Drug Metabolism and Disposition, Vol. 27, No. 9, 1999, p. 1085-1091.

Research output: Contribution to journalArticle

Molnár, P. ; Fekete, I. ; Schlageter, Kurt E. ; Lapin, Gregory D. ; Groothuis, Dennis R. / Absence of host-site influence on angiogenesis, blood flow, and permeability in transplanted RG-2 gliomas. In: Drug Metabolism and Disposition. 1999 ; Vol. 27, No. 9. pp. 1085-1091.
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