Absence of collagen XVIII in mice causes age-related insufficiency in retinal pigment epithelium proteostasis

Niko Kivinen, S. Felszeghy, Aino I. Kinnunen, Niko Setälä, Mari Aikio, Kati Kinnunen, Reijo Sironen, Taina Pihlajaniemi, Anu Kauppinen, Kai Kaarniranta

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6 Citations (Scopus)

Abstract

Collagen XVIII has the structural properties of both collagen and proteoglycan. It has been found at the basement membrane/stromal interface where it is thought to mediate their attachment. Endostatin, a proteolytic fragment from collagen XVIII C-terminal end has been reported to possess anti-angiogenic properties. Age-related vision loss in collagen XVIII mutant mice has been accompanied with a pathological accumulation of deposits under the retinal pigment epithelium (RPE). We have recently demonstrated that impaired proteasomal and autophagy clearance are associated with the pathogenesis of age-related macular degeneration. This study examined the staining levels of proteasomal and autophagy markers in the RPE of different ages of the Col18a1−/− mice. Eyes from 3, 6–7, 10–13 and 18 months old mice were enucleated and embedded in paraffin according to the routine protocol. Sequential 5 μm-thick parasagittal samples were immunostained for proteasome and autophagy markers ubiquitin (ub), SQSTM1/p62 and beclin-1. The levels of immunopositivity in the RPE cells were evaluated by confocal microscopy. Collagen XVIII knock-out mice had undergone age-related RPE degeneration accompanied by an accumulation of drusen-like deposits. Ub protein conjugate staining was prominent in both RPE cytoplasm and extracellular space whereas SQSTM1/p62 and beclin-1 stainings were clearly present in the basal part of RPE cell cytoplasm in the Col18a1−/− mice. SQSTM1/p62 displayed mild extracellular space staining. Disturbed proteostasis regulated by collagen XVIII might be responsible for the RPE degeneration, increased protein aggregation, ultimately leading to choroidal neovascularization.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalBiogerontology
DOIs
Publication statusAccepted/In press - Apr 28 2016

Keywords

  • Aging
  • Animal model
  • Autophagy
  • Collagen XVIII
  • Macula
  • Retina

ASJC Scopus subject areas

  • Geriatrics and Gerontology
  • Gerontology
  • Ageing

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    Kivinen, N., Felszeghy, S., Kinnunen, A. I., Setälä, N., Aikio, M., Kinnunen, K., Sironen, R., Pihlajaniemi, T., Kauppinen, A., & Kaarniranta, K. (Accepted/In press). Absence of collagen XVIII in mice causes age-related insufficiency in retinal pigment epithelium proteostasis. Biogerontology, 1-13. https://doi.org/10.1007/s10522-016-9647-7