Abscisic acid coordinates nod factor and cytokinin signaling during the regulation of nodulation in Medicago truncatula

Yiliang Ding, P. Kaló, Craig Yendrek, Jongho Sun, Yan Liang, John F. Marsh, Jeanne M. Harris, Giles E D Oldroyd

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Nodulation is tightly regulated in legumes to ensure appropriate levels of nitrogen fixation without excessive depletion of carbon reserves. This balance is maintained by intimately linking nodulation and its regulation with plant hormones. It has previously been shown that ethylene and jasmonic acid (JA) are able to regulate nodulation and Nod factor signal transduction. Here, we characterize the nature of abscisic acid (ABA) regulation of nodulation. We show that application of ABA inhibits nodulation, bacterial infection, and nodulin gene expression in Medicago truncatula. ABA acts in a similar manner as JA and ethylene, regulating Nod factor signaling and affecting the nature of Nod factor-induced calcium spiking. However, this action is independent of the ethylene signal transduction pathway. We show that genetic inhibition of ABA signaling through the use of a dominant-negative allele of ABSCISIC ACID INSENSITIVE1 leads to a hypernodulation phenotype. In addition, we characterize a novel locus of M. truncatula, SENSITIVITY TO ABA, that dictates the sensitivity of the plant to ABA and, as such, impacts the regulation of nodulation. We show that ABA can suppress Nod factor signal transduction in the epidermis and can regulate cytokinin induction of the nodule primordium in the root cortex. Therefore, ABA is capable of coordinately regulating the diverse developmental pathways associated with nodule formation and can intimately dictate the nature of the plants' response to the symbiotic bacteria.

Original languageEnglish
Pages (from-to)2681-2695
Number of pages15
JournalPlant Cell
Volume20
Issue number10
DOIs
Publication statusPublished - Oct 2008

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Medicago truncatula
Cytokinins
Abscisic Acid
nodulation
cytokinins
abscisic acid
signal transduction
ethylene
Signal Transduction
jasmonic acid
nodulins
root primordia
Nitrogen Fixation
Plant Growth Regulators
epidermis (plant)
bacterial infections
Bacterial Infections
Epidermis
nitrogen fixation
Fabaceae

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Abscisic acid coordinates nod factor and cytokinin signaling during the regulation of nodulation in Medicago truncatula. / Ding, Yiliang; Kaló, P.; Yendrek, Craig; Sun, Jongho; Liang, Yan; Marsh, John F.; Harris, Jeanne M.; Oldroyd, Giles E D.

In: Plant Cell, Vol. 20, No. 10, 10.2008, p. 2681-2695.

Research output: Contribution to journalArticle

Ding, Y, Kaló, P, Yendrek, C, Sun, J, Liang, Y, Marsh, JF, Harris, JM & Oldroyd, GED 2008, 'Abscisic acid coordinates nod factor and cytokinin signaling during the regulation of nodulation in Medicago truncatula', Plant Cell, vol. 20, no. 10, pp. 2681-2695. https://doi.org/10.1105/tpc.108.061739
Ding, Yiliang ; Kaló, P. ; Yendrek, Craig ; Sun, Jongho ; Liang, Yan ; Marsh, John F. ; Harris, Jeanne M. ; Oldroyd, Giles E D. / Abscisic acid coordinates nod factor and cytokinin signaling during the regulation of nodulation in Medicago truncatula. In: Plant Cell. 2008 ; Vol. 20, No. 10. pp. 2681-2695.
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