Abnormal protein patterns in blood serum and cerebrospinal fluid detected by capillary electrophoresis

Mariella Ivanova, Elena Tzvetanova, Veska Jetcheva, Ferenc Kilár

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Capillary zone electrophoresis and high-resolution agarose gel electrophoresis were compared to detect protein components in serum and cerebrospinal fluid of patients. Both electrophoretic methods proved to be useful for detection of protein abnormalities (e.g., mono- and oligoclonal bands) in biological fluids, but capillary electrophoresis offered several important advantages, such as sample application without preliminary concentration, lack of staining procedures, and on-line evaluation of patterns. Furthermore, capillary electrophoresis exhibits shorter analysis time and high resolution with low baseline noise. The results were proven to be powerful in diagnosis and monitoring of dyscrasias in routine laboratory practice.

Original languageEnglish
Pages (from-to)141-150
Number of pages10
JournalJournal of biochemical and biophysical methods
Volume53
Issue number1-3
DOIs
Publication statusPublished - Oct 1 2002

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Keywords

  • Blood serum
  • Capillary zone electrophoresis
  • Cerebrospinal fluid
  • Oligoclonal bands
  • Proteins

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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