Abiotic stress-induced changes in glutathione and thioredoxin h levels in maize

G. Kocsy, Károly Kobrehel, G. Szalai, Marie Pierre Duviau, Zoltán Buzás, G. Galiba

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The possible relationship between abiotic stress-induced changes in glutathione (GSH) and thioredoxin h (Trx h) levels was investigated in two maize (Zea mais L.) inbred lines. Less damage was suffered by the chilling-tolerant line Z7 during chilling and by the chilling-sensitive line Penjalinan (Pen) during heat stress, as shown by electrolyte leakage, dry weight and water content data. These parameters were similar in both genotypes during polyethylene glycol (PEG) treatment. Pretreatment with abscisic acid (ABA) reduced the heat-induced increase in electrolyte leakage. A great increase in the GSH and Trx h levels was induced by chilling in Z7 and, conversely, by heat stress in Pen. Extreme temperatures had a similar effect on the level of cysteine and γ-glutamylcysteine (GSH precursors) and on the ratio of reduced to oxidised forms of the three low-molecular-weight thiols, as well as on the activity of glutathione reductase (EC 1.6.4.2). ABA and PEG treatment did not change the GSH content or even decreased it, but increased the Trx h level in both genotypes. A combination of ABA or PEG treatment with heat stress induced similar changes in the GSH and Trx h contents to those induced by heat stress alone. The parallel changes in GSH and Trx h levels at extreme temperatures, and the opposite changes observed during ABA and PEG treatment indicate the stress-specific regulation of their levels during various abiotic stresses.

Original languageEnglish
Pages (from-to)101-112
Number of pages12
JournalEnvironmental and Experimental Botany
Volume52
Issue number2
DOIs
Publication statusPublished - Oct 2004

Fingerprint

polyethylene glycol
abiotic stress
heat stress
abscisic acid
glutathione
maize
corn
glutathione-disulfide reductase
electrolytes
genotype
electrolyte
leakage
thiols
inbred lines
cysteine
temperature
pretreatment
Zea mays
molecular weight
water content

Keywords

  • Abscisic acid
  • Extreme temperatures
  • Glutathione
  • Osmotic stress
  • Thioredoxin h
  • Zea

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Environmental Science(all)

Cite this

Abiotic stress-induced changes in glutathione and thioredoxin h levels in maize. / Kocsy, G.; Kobrehel, Károly; Szalai, G.; Duviau, Marie Pierre; Buzás, Zoltán; Galiba, G.

In: Environmental and Experimental Botany, Vol. 52, No. 2, 10.2004, p. 101-112.

Research output: Contribution to journalArticle

Kocsy, G. ; Kobrehel, Károly ; Szalai, G. ; Duviau, Marie Pierre ; Buzás, Zoltán ; Galiba, G. / Abiotic stress-induced changes in glutathione and thioredoxin h levels in maize. In: Environmental and Experimental Botany. 2004 ; Vol. 52, No. 2. pp. 101-112.
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