ABCG2 (breast cancer resistance protein/mitoxantrone resistance-associated protein) ATPase assay: A useful tool to detect drug-transporter interactions

Hristos Glavinas, Emese Kis, Ákos Pál, Rita Kovács, Márton Jani, Erika Vági, Éva Molnár, Száva Bánsághi, Z. Kele, T. Janáky, G. Báthori, Oliver Von Richter, Gerrit Jan Koomen, Péter Krajcsi

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The ATPase assay using membrane preparations from recombinant baculovirus-infected Spodoptera frugiperda ovarian (Sf9) cells is widely used to detect the interaction of compounds with different ATP-binding cassette transporters. However, Sf9 membrane preparations containing the wild-type ABCG2 transporter show an elevated baseline vanadate-sensitive ATPase activity, which cannot be further stimulated by substrates of ABCG2. Therefore, this assay system cannot be used for the detection of ABCG2 substrates. To overcome this difficulty we 1) purified membranes from a selected human cell line expressing wild-type ABCG2, and 2) inhibited the baseline ATPase activity with different inhibitors. In our modified assay, ABCG2 substrates were able to stimulate the baseline ATPase activity of ABCG2 expressed in membranes of human cells. Furthermore, using the specific ABCG2 inhibitors Ko143 or Ko134 allowed us to suppress the baseline vanadate-sensitive ATPase activity. Substrates of ABCG2 could stimulate this suppressed baseline ATPase, resulting in a better signal-to-background ratio and a robust assay to detect substrates of the ABCG2 transporter. The ATPase assay and the direct vesicular transport measurements for estrone-3-sulfate were in good accordance.

Original languageEnglish
Pages (from-to)1533-1542
Number of pages10
JournalDrug Metabolism and Disposition
Volume35
Issue number9
DOIs
Publication statusPublished - Sep 2007

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Drug interactions
Mitoxantrone
Drug Interactions
Adenosine Triphosphatases
Assays
Breast Neoplasms
Membranes
Substrates
Proteins
Cells
Sf9 Cells
Spodoptera
ATP-Binding Cassette Transporters
Baculoviridae
Cell Membrane
Cell Line

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

ABCG2 (breast cancer resistance protein/mitoxantrone resistance-associated protein) ATPase assay : A useful tool to detect drug-transporter interactions. / Glavinas, Hristos; Kis, Emese; Pál, Ákos; Kovács, Rita; Jani, Márton; Vági, Erika; Molnár, Éva; Bánsághi, Száva; Kele, Z.; Janáky, T.; Báthori, G.; Von Richter, Oliver; Koomen, Gerrit Jan; Krajcsi, Péter.

In: Drug Metabolism and Disposition, Vol. 35, No. 9, 09.2007, p. 1533-1542.

Research output: Contribution to journalArticle

Glavinas, H, Kis, E, Pál, Á, Kovács, R, Jani, M, Vági, E, Molnár, É, Bánsághi, S, Kele, Z, Janáky, T, Báthori, G, Von Richter, O, Koomen, GJ & Krajcsi, P 2007, 'ABCG2 (breast cancer resistance protein/mitoxantrone resistance-associated protein) ATPase assay: A useful tool to detect drug-transporter interactions', Drug Metabolism and Disposition, vol. 35, no. 9, pp. 1533-1542. https://doi.org/10.1124/dmd.106.014605
Glavinas, Hristos ; Kis, Emese ; Pál, Ákos ; Kovács, Rita ; Jani, Márton ; Vági, Erika ; Molnár, Éva ; Bánsághi, Száva ; Kele, Z. ; Janáky, T. ; Báthori, G. ; Von Richter, Oliver ; Koomen, Gerrit Jan ; Krajcsi, Péter. / ABCG2 (breast cancer resistance protein/mitoxantrone resistance-associated protein) ATPase assay : A useful tool to detect drug-transporter interactions. In: Drug Metabolism and Disposition. 2007 ; Vol. 35, No. 9. pp. 1533-1542.
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