ABCC6 is a basolateral plasma membrane protein

Viola Pomozi, Olivier Le Saux, Christopher Brampton, Ailea Apana, Attila Iliás, Flóra Szeri, Ludovic Martin, K. Monostory, S. Paku, B. Sarkadi, G. Szakács, A. Váradi

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

RATIONALE:: ABCC6 plays a crucial role in ectopic calcification; mutations of the gene cause pseudoxanthoma elasticum and general arterial calcification of infancy. To elucidate the role of ABCC6 in cellular physiology and disease, it is crucial to establish the exact subcellular localization of the native ABCC6 protein. OBJECTIVE:: In a recent article in Circulation Research, ABCC6 was reported to localize to the mitochondria-associated membrane and not the plasma membrane. As the suggested mitochondrial localization is inconsistent with published data and the presumed role of ABCC6, we performed experiments to determine the cellular localization of ABCC6 in its physiological environment. METHODS AND RESULTS:: We performed immunofluorescent labeling of frozen mouse and human liver sections, as well as primary hepatocytes. We used several different antibodies recognizing human and mouse ABCC6. Our results unequivocally show that ABCC6 is in the basolateral membrane of hepatocytes and is not associated with the mitochondria, mitochondria-associated membrane, or the endoplasmic reticulum. CONCLUSIONS:: Our findings support the model that ABCC6 is in the basolateral membrane, mediating the sinusoidal efflux of a metabolite from the hepatocytes to systemic circulation.

Original languageEnglish
JournalCirculation Research
Volume112
Issue number11
DOIs
Publication statusPublished - May 24 2013

Fingerprint

Blood Proteins
Membrane Proteins
Cell Membrane
Hepatocytes
Mitochondria
Membranes
Pseudoxanthoma Elasticum
Endoplasmic Reticulum
Mutation
Antibodies
Liver
Research
Genes
Proteins

Keywords

  • arterial calcification
  • generalized, of infancy
  • hepatocytes
  • pseudoxanthoma elasticum soft tissue calcification

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Pomozi, V., Le Saux, O., Brampton, C., Apana, A., Iliás, A., Szeri, F., ... Váradi, A. (2013). ABCC6 is a basolateral plasma membrane protein. Circulation Research, 112(11). https://doi.org/10.1161/CIRCRESAHA.111.300194

ABCC6 is a basolateral plasma membrane protein. / Pomozi, Viola; Le Saux, Olivier; Brampton, Christopher; Apana, Ailea; Iliás, Attila; Szeri, Flóra; Martin, Ludovic; Monostory, K.; Paku, S.; Sarkadi, B.; Szakács, G.; Váradi, A.

In: Circulation Research, Vol. 112, No. 11, 24.05.2013.

Research output: Contribution to journalArticle

Pomozi V, Le Saux O, Brampton C, Apana A, Iliás A, Szeri F et al. ABCC6 is a basolateral plasma membrane protein. Circulation Research. 2013 May 24;112(11). https://doi.org/10.1161/CIRCRESAHA.111.300194
Pomozi, Viola ; Le Saux, Olivier ; Brampton, Christopher ; Apana, Ailea ; Iliás, Attila ; Szeri, Flóra ; Martin, Ludovic ; Monostory, K. ; Paku, S. ; Sarkadi, B. ; Szakács, G. ; Váradi, A. / ABCC6 is a basolateral plasma membrane protein. In: Circulation Research. 2013 ; Vol. 112, No. 11.
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