ABCC6 expression is regulated by CCAAT/enhancer-binding protein activating a primate-specific sequence located in the first intron of the gene

Marcin Ratajewski, Hugues De Boussac, Iwona Sachrajda, Caroline Bacquet, T. Kovács, A. Váradi, Lukasz Pulaski, T. Arányi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Pseudoxanthoma elasticum (PXE), a rare recessive genetic disease causing skin, eye, and cardiovascular lesions, is characterized by the calcification of elastic fibers. The disorder is due to loss-of-function mutations of the ABCC6 gene, but the pathophysiology of the disease is still not understood. Here we investigated the transcriptional regulation of the gene, using DNase I hypersensitivity assay followed by luciferase reporter gene assay. We identified three DNase I hypersensitive sites (HSs) specific to cell lines expressing ABCC6. These HSs are located in the proximal promoter and in the first intron of the gene. We further characterized the role of the HSs by luciferase assay and demonstrated the transcriptional activity of the intronic HS. We identified the CCAAT/enhancer-binding protein β (C/EBPβ) as a factor binding the second intronic HS by chromatin immunoprecipitation and corroborated this finding by luciferase assays. We also showed that C/EBPβ interacts with the proximal promoter of the gene. We propose that C/EBPβ forms a complex with other regulatory proteins including the previously identified regulatory factor hepatocyte nuclear factor 4α (HNF4α). This complex would account for the tissue-specific expression of the gene and might serve as a metabolic sensor. Our results point toward a better understanding of the physiological role of ABCC6.

Original languageEnglish
Pages (from-to)2709-2717
Number of pages9
JournalJournal of Investigative Dermatology
Volume132
Issue number12
DOIs
Publication statusPublished - Dec 2012

Fingerprint

CCAAT-Enhancer-Binding Proteins
Introns
Primates
Genes
Luciferases
Deoxyribonuclease I
Assays
Genetic Skin Diseases
Hepatocyte Nuclear Factor 4
Pseudoxanthoma Elasticum
Elastic Tissue
Chromatin Immunoprecipitation
Reporter Genes
Hypersensitivity
Rubber
Gene Expression
Cell Line
Mutation
Chromatin
Skin

ASJC Scopus subject areas

  • Dermatology
  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

ABCC6 expression is regulated by CCAAT/enhancer-binding protein activating a primate-specific sequence located in the first intron of the gene. / Ratajewski, Marcin; De Boussac, Hugues; Sachrajda, Iwona; Bacquet, Caroline; Kovács, T.; Váradi, A.; Pulaski, Lukasz; Arányi, T.

In: Journal of Investigative Dermatology, Vol. 132, No. 12, 12.2012, p. 2709-2717.

Research output: Contribution to journalArticle

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abstract = "Pseudoxanthoma elasticum (PXE), a rare recessive genetic disease causing skin, eye, and cardiovascular lesions, is characterized by the calcification of elastic fibers. The disorder is due to loss-of-function mutations of the ABCC6 gene, but the pathophysiology of the disease is still not understood. Here we investigated the transcriptional regulation of the gene, using DNase I hypersensitivity assay followed by luciferase reporter gene assay. We identified three DNase I hypersensitive sites (HSs) specific to cell lines expressing ABCC6. These HSs are located in the proximal promoter and in the first intron of the gene. We further characterized the role of the HSs by luciferase assay and demonstrated the transcriptional activity of the intronic HS. We identified the CCAAT/enhancer-binding protein β (C/EBPβ) as a factor binding the second intronic HS by chromatin immunoprecipitation and corroborated this finding by luciferase assays. We also showed that C/EBPβ interacts with the proximal promoter of the gene. We propose that C/EBPβ forms a complex with other regulatory proteins including the previously identified regulatory factor hepatocyte nuclear factor 4α (HNF4α). This complex would account for the tissue-specific expression of the gene and might serve as a metabolic sensor. Our results point toward a better understanding of the physiological role of ABCC6.",
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