ABC transporter ATPase of Chlamydophila pneumoniae as a potential vaccine candidate

Ágnes Szabó, Zoltán Sipák, A. Miczák, Ildikó Faludi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Better vaccines and new therapeutic drugs could be a successful breakthrough against intracellular bacteria. M. tuberculosis ABC transporter ATPase (Rv0986) plays a role in mycobacterial virulence by inhibiting phagosome-lysosome fusion. Thus, it could be a potential vaccine candidate. C. pneumoniae another important intracellular bacterium possesses a protein named CpB0255, which is homologous with the mycobacterial Rv0986. The aim of this study was the cloning, over-expression and purification of CpB0255 ABC transporter ATPase protein to study its biological properties. The immunogenicity and protective effect of recombinant chlamydial ATPase protein combined with Alum adjuvant were investigated in mice. The immunization resulted in the reduction of the number of viable C. pneumoniae in the lungs after challenge. Our results confirm that chlamydial ATPase induces protective immunity in mice.

Original languageEnglish
Pages (from-to)11-20
Number of pages10
JournalActa Microbiologica et Immunologica Hungarica
Volume60
Issue number1
DOIs
Publication statusPublished - Mar 1 2013

Fingerprint

Chlamydophila pneumoniae
ATP-Binding Cassette Transporters
Adenosine Triphosphatases
Vaccines
Bacteria
Phagosomes
Proteins
Lysosomes
Virulence
Organism Cloning
Immunity
Immunization
Tuberculosis
Lung
Pharmaceutical Preparations
Therapeutics

Keywords

  • ABC transporter ATPase
  • CpB0255
  • over-expression

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

ABC transporter ATPase of Chlamydophila pneumoniae as a potential vaccine candidate. / Szabó, Ágnes; Sipák, Zoltán; Miczák, A.; Faludi, Ildikó.

In: Acta Microbiologica et Immunologica Hungarica, Vol. 60, No. 1, 01.03.2013, p. 11-20.

Research output: Contribution to journalArticle

Szabó, Ágnes ; Sipák, Zoltán ; Miczák, A. ; Faludi, Ildikó. / ABC transporter ATPase of Chlamydophila pneumoniae as a potential vaccine candidate. In: Acta Microbiologica et Immunologica Hungarica. 2013 ; Vol. 60, No. 1. pp. 11-20.
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