A Viral Phospholipase A2 Is Required for Parvovirus Infectivity

Zoltán Zádori, József Szelei, Marie Claude Lacoste, Yi Li, Sébastien Gariépy, Philippe Raymond, Marc Allaire, Ivan R. Nabi, Peter Tijssen

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Abstract

Sequence analysis revealed phospholipase A2 (PLA2) motifs in capsid proteins of parvoviruses. Although PLA2 activity is not known to exist in viruses, putative PLA2S from divergent parvoviruses, human B19, porcine parvovirus, and insect GmDNV (densovirus from Galleria mellonella), can emulate catalytic properties of secreted PLA2. Mutations of critical amino acids strongly reduce both PLA2 activity and, proportionally, viral infectivity, but cell surface attachment, entry, and endocytosis by PLA2-deficient virions are not affected. PLA2 activity is critical for efficient transfer of the viral genome from late endosomes/lysosomes to the nucleus to initiate replication. These findings offer the prospect of developing PLA2 inhibitors as a new class of antiviral drugs against parvovirus infections and associated diseases.

Original languageEnglish
Pages (from-to)291-302
Number of pages12
JournalDevelopmental Cell
Volume1
Issue number2
DOIs
Publication statusPublished - Aug 2001

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ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

Cite this

Zádori, Z., Szelei, J., Lacoste, M. C., Li, Y., Gariépy, S., Raymond, P., Allaire, M., Nabi, I. R., & Tijssen, P. (2001). A Viral Phospholipase A2 Is Required for Parvovirus Infectivity. Developmental Cell, 1(2), 291-302. https://doi.org/10.1016/S1534-5807(01)00031-4