A tradeoff between protein stability and conformational mobility in homotrimeric dUTPases

Eniko Takács, Vince K. Grolmusz, B. Vértessy

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Oligomerization directs active site formation in homotrimeric 2 -deoxyuridine triphosphate pyrophosphatases (dUTPases). Stability of the homotrimer is a central determinant in enzyme function. The present comparative studies of bacterial and fruitfly dUTPases with homologous 3D structures by differential scanning microcalorimetry; fluorescence, circular dichorism and infrared spectroscopies, demonstrate that unfolding is a two-state highly cooperative transition in both dUTPases excluding a significantly populated intermediate state of dissociated and folded monomers. The eukaryotic protein is much less resistant against either thermal or guanidine hydrochloride-induced denaturation. Results suggest that hydrophobic packing of the inner threefold channel of the dUTPase homotrimer greatly contributes to stability.

Original languageEnglish
Pages (from-to)48-54
Number of pages7
JournalFEBS Letters
Volume566
Issue number1-3
DOIs
Publication statusPublished - May 21 2004

Fingerprint

Pyrophosphatases
Protein Stability
Oligomerization
Proteins
Denaturation
Guanidine
Infrared spectroscopy
Catalytic Domain
Spectrum Analysis
Hot Temperature
Monomers
Fluorescence
Scanning
Enzymes
deoxyuridine triphosphate

Keywords

  • α,β-Imino-dUTP, 2-deoxyuridine 5 -(α,β-imino)triphosphate
  • CD, circular dichroism
  • DSC, differential scanning calorimetry
  • dUTPase, dUTP pyrophosphatase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A tradeoff between protein stability and conformational mobility in homotrimeric dUTPases. / Takács, Eniko; Grolmusz, Vince K.; Vértessy, B.

In: FEBS Letters, Vol. 566, No. 1-3, 21.05.2004, p. 48-54.

Research output: Contribution to journalArticle

Takács, Eniko ; Grolmusz, Vince K. ; Vértessy, B. / A tradeoff between protein stability and conformational mobility in homotrimeric dUTPases. In: FEBS Letters. 2004 ; Vol. 566, No. 1-3. pp. 48-54.
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