A topographic quantitative method for measuring brain tissue pH under physiological and pathophysiological conditions

L. Csiba, W. Paschen, K. A. Hossmann

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A technique was developed for the quantitative regional assessment of brain pH by a modification of the umbelliferone method. Twenty micron thick sections were brought into contact with umbelliferone-soaked paper strips and the fluorescence (450 nm) following excitation at 370 nm and 340 nm was recorded photographically. The 340 nm excitation image was subtracted from 370 nm picture, using a computerized image-processing system. Regional pH values were measured in rats and cats under normal and ischemic conditions.

Original languageEnglish
Pages (from-to)334-337
Number of pages4
JournalBrain research
Issue number1-2
Publication statusPublished - Dec 19 1983



  • brain pH
  • cats
  • cerebral ischemia
  • rats
  • umbelliferone fluorescence

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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