A tightly bound chromosome antigen is detected by monoclonal antibodies in a ring-like structure on human centromeres

Katalin A. Holland, Judit Keresõ, József Zákány, Tünde Pravnovskzy, E. Monostori, N. Belyaer, Gyula Hadlaczky

Research output: Contribution to journalArticle

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Abstract

Monoclonal antibodies (Mabs) were raised against isolated Chinese hamster protein-depleted chromosomes Chromosome scaffolds) in order to probe for components involved in the higher-order structure of mammalian chromosomes. One of the Mabs detected a ring-like structure in metaphase at the centromere, which is conserved between Chinese hamster and human cells. Additionally, the Mab stained the centrioles in interphase cells in these two species. The antigen was enriched in chromosomal protein preparations by comparison with nuclear protein samples, and has an apparent Mr=170,000. The centromere antigen remained present in chromosome scaffold preparations, indicating that it was tightly associated with DNA. The antigen was distinct in its centromeric localisation from any of the centromere antigens reported to date. A possible role of the antigen in stabilising the centromere, by holding the sister chromatids together until their separation at the metaphase-anaphase transition is presented.

Original languageEnglish
Pages (from-to)559-566
Number of pages8
JournalChromosoma
Volume103
Issue number8
DOIs
Publication statusPublished - Feb 1995

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Centromere
Chromosomes
Monoclonal Antibodies
Antigens
Metaphase
Cricetulus
Mammalian Chromosomes
Centrioles
Anaphase
Chromatids
Interphase
Nuclear Proteins
Proteins
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Holland, K. A., Keresõ, J., Zákány, J., Pravnovskzy, T., Monostori, E., Belyaer, N., & Hadlaczky, G. (1995). A tightly bound chromosome antigen is detected by monoclonal antibodies in a ring-like structure on human centromeres. Chromosoma, 103(8), 559-566. https://doi.org/10.1007/BF00355321

A tightly bound chromosome antigen is detected by monoclonal antibodies in a ring-like structure on human centromeres. / Holland, Katalin A.; Keresõ, Judit; Zákány, József; Pravnovskzy, Tünde; Monostori, E.; Belyaer, N.; Hadlaczky, Gyula.

In: Chromosoma, Vol. 103, No. 8, 02.1995, p. 559-566.

Research output: Contribution to journalArticle

Holland, KA, Keresõ, J, Zákány, J, Pravnovskzy, T, Monostori, E, Belyaer, N & Hadlaczky, G 1995, 'A tightly bound chromosome antigen is detected by monoclonal antibodies in a ring-like structure on human centromeres', Chromosoma, vol. 103, no. 8, pp. 559-566. https://doi.org/10.1007/BF00355321
Holland, Katalin A. ; Keresõ, Judit ; Zákány, József ; Pravnovskzy, Tünde ; Monostori, E. ; Belyaer, N. ; Hadlaczky, Gyula. / A tightly bound chromosome antigen is detected by monoclonal antibodies in a ring-like structure on human centromeres. In: Chromosoma. 1995 ; Vol. 103, No. 8. pp. 559-566.
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