A systematic approach to selenium speciation in selenized yeast

Aleksandra Polatajko, Magdalena Śliwka-Kaszyńska, M. Dernovics, Rafal Ruzik, Jorge Ruiz Encinar, Joanna Szpunar

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

A systematic approach to the characterization of selenized yeast supplements in terms of the speciation of selenium was developed. The optimized fractionation procedures included the sequential leaching of water soluble, cell-wall bound, and membrane-protein selenium followed by a further fractionation of each extract by high-resolution size-exclusion chromatography. The stability of fractions collected as Chromatographic peaks was investigated in the presence of a proteolytic enzyme (pronase XIV) and trypsin in order to discriminate between selenium-containing peptides and other selenocompounds. Reversed-phase HPLC of tryptic digests of size-exclusion Chromatographic fractions allowed the identification of selenopeptides by MALDI and electrospray MS. The complexity of the speciation of the water-soluble selenium in yeast was confirmed. Surprisingly, selenomethionine in the water insoluble fraction was found to be bound physically to cell wall constituents rather than being incorporated chemically into the protein structure, in contrast to former studies.

Original languageEnglish
Pages (from-to)114-120
Number of pages7
JournalJournal of Analytical Atomic Spectrometry
Volume19
Issue number1
DOIs
Publication statusPublished - 2004

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Selenium
Yeast
Fractionation
Water
Selenomethionine
Pronase
Size exclusion chromatography
Trypsin
Leaching
Membrane Proteins
Peptide Hydrolases
Cells
Peptides
Proteins

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Polatajko, A., Śliwka-Kaszyńska, M., Dernovics, M., Ruzik, R., Encinar, J. R., & Szpunar, J. (2004). A systematic approach to selenium speciation in selenized yeast. Journal of Analytical Atomic Spectrometry, 19(1), 114-120. https://doi.org/10.1039/b308756p

A systematic approach to selenium speciation in selenized yeast. / Polatajko, Aleksandra; Śliwka-Kaszyńska, Magdalena; Dernovics, M.; Ruzik, Rafal; Encinar, Jorge Ruiz; Szpunar, Joanna.

In: Journal of Analytical Atomic Spectrometry, Vol. 19, No. 1, 2004, p. 114-120.

Research output: Contribution to journalArticle

Polatajko, A, Śliwka-Kaszyńska, M, Dernovics, M, Ruzik, R, Encinar, JR & Szpunar, J 2004, 'A systematic approach to selenium speciation in selenized yeast', Journal of Analytical Atomic Spectrometry, vol. 19, no. 1, pp. 114-120. https://doi.org/10.1039/b308756p
Polatajko, Aleksandra ; Śliwka-Kaszyńska, Magdalena ; Dernovics, M. ; Ruzik, Rafal ; Encinar, Jorge Ruiz ; Szpunar, Joanna. / A systematic approach to selenium speciation in selenized yeast. In: Journal of Analytical Atomic Spectrometry. 2004 ; Vol. 19, No. 1. pp. 114-120.
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