A synthetic corticosteroid, dexamethasone regulates generation of soluble form of interleukin-6 receptor of human lymphocytes, in vitro

Anna Polgár, Márta Brózik, S. Tóth, Marcsilla Holub, A. Falus

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In contrast to most of the soluble cytokine receptor antagonists properties, the soluble IL-6 receptor (sIL-6R) occurring in various body fluids of healthy persons and patients with various diseases is an agonist. The enhancing effect is due to its ability to form complex with IL-6 and to bind to gp130 making constitutively IL-6 receptor negative cells responsive for IL-6. The generation as well as the functional role of soluble IL-6 receptor is poorly understood. Earlier, we found that the sIL-6R levels in sera of patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) were higher than those of the control group measured by ELISA sandwich technology. In the present study we detected different levels of sIL-6R in the supernatants of lymphocyte cultures of healthy persons and patients with RA as well as SLE. Moreover, we found, that in vitro dexamethasone treatment stimulated generation of sIL-6R in both healthy persons and in active SLE, while it strongly suppressed production of sIL-6R in both RA groups. At mRNA level, we found that in SLE both the IL-6R mRNA encoding the membrane spanning and alternatively spliced (soluble) variants increased. Surprisingly, the strong decrease of sIL6R protein in RA was not found at mRNA level.

Original languageEnglish
Pages (from-to)307-315
Number of pages9
JournalActa Biologica Hungarica
Volume53
Issue number3
DOIs
Publication statusPublished - 2002

Fingerprint

Interleukin-6 Receptors
body fluid
functional role
Lymphocytes
adrenal cortex hormones
dexamethasone
interleukin-6
Dexamethasone
serum
Adrenal Cortex Hormones
lymphocytes
membrane
receptors
protein
lupus erythematosus
Systemic Lupus Erythematosus
rheumatoid arthritis
Rheumatoid Arthritis
Messenger RNA
Interleukin-6

Keywords

  • Dexamethasone
  • Rheumatoid arthritis (RA)
  • Soluble interleukin-6 receptor (sIL-6R)
  • Systemic lupus erythematosus (SLE)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

A synthetic corticosteroid, dexamethasone regulates generation of soluble form of interleukin-6 receptor of human lymphocytes, in vitro. / Polgár, Anna; Brózik, Márta; Tóth, S.; Holub, Marcsilla; Falus, A.

In: Acta Biologica Hungarica, Vol. 53, No. 3, 2002, p. 307-315.

Research output: Contribution to journalArticle

@article{344b676226484fbb8eb74a76439c453c,
title = "A synthetic corticosteroid, dexamethasone regulates generation of soluble form of interleukin-6 receptor of human lymphocytes, in vitro",
abstract = "In contrast to most of the soluble cytokine receptor antagonists properties, the soluble IL-6 receptor (sIL-6R) occurring in various body fluids of healthy persons and patients with various diseases is an agonist. The enhancing effect is due to its ability to form complex with IL-6 and to bind to gp130 making constitutively IL-6 receptor negative cells responsive for IL-6. The generation as well as the functional role of soluble IL-6 receptor is poorly understood. Earlier, we found that the sIL-6R levels in sera of patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) were higher than those of the control group measured by ELISA sandwich technology. In the present study we detected different levels of sIL-6R in the supernatants of lymphocyte cultures of healthy persons and patients with RA as well as SLE. Moreover, we found, that in vitro dexamethasone treatment stimulated generation of sIL-6R in both healthy persons and in active SLE, while it strongly suppressed production of sIL-6R in both RA groups. At mRNA level, we found that in SLE both the IL-6R mRNA encoding the membrane spanning and alternatively spliced (soluble) variants increased. Surprisingly, the strong decrease of sIL6R protein in RA was not found at mRNA level.",
keywords = "Dexamethasone, Rheumatoid arthritis (RA), Soluble interleukin-6 receptor (sIL-6R), Systemic lupus erythematosus (SLE)",
author = "Anna Polg{\'a}r and M{\'a}rta Br{\'o}zik and S. T{\'o}th and Marcsilla Holub and A. Falus",
year = "2002",
doi = "10.1556/ABiol.53.2002.3.7",
language = "English",
volume = "53",
pages = "307--315",
journal = "Acta Biologica Hungarica",
issn = "0236-5383",
publisher = "Akademiai Kiado",
number = "3",

}

TY - JOUR

T1 - A synthetic corticosteroid, dexamethasone regulates generation of soluble form of interleukin-6 receptor of human lymphocytes, in vitro

AU - Polgár, Anna

AU - Brózik, Márta

AU - Tóth, S.

AU - Holub, Marcsilla

AU - Falus, A.

PY - 2002

Y1 - 2002

N2 - In contrast to most of the soluble cytokine receptor antagonists properties, the soluble IL-6 receptor (sIL-6R) occurring in various body fluids of healthy persons and patients with various diseases is an agonist. The enhancing effect is due to its ability to form complex with IL-6 and to bind to gp130 making constitutively IL-6 receptor negative cells responsive for IL-6. The generation as well as the functional role of soluble IL-6 receptor is poorly understood. Earlier, we found that the sIL-6R levels in sera of patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) were higher than those of the control group measured by ELISA sandwich technology. In the present study we detected different levels of sIL-6R in the supernatants of lymphocyte cultures of healthy persons and patients with RA as well as SLE. Moreover, we found, that in vitro dexamethasone treatment stimulated generation of sIL-6R in both healthy persons and in active SLE, while it strongly suppressed production of sIL-6R in both RA groups. At mRNA level, we found that in SLE both the IL-6R mRNA encoding the membrane spanning and alternatively spliced (soluble) variants increased. Surprisingly, the strong decrease of sIL6R protein in RA was not found at mRNA level.

AB - In contrast to most of the soluble cytokine receptor antagonists properties, the soluble IL-6 receptor (sIL-6R) occurring in various body fluids of healthy persons and patients with various diseases is an agonist. The enhancing effect is due to its ability to form complex with IL-6 and to bind to gp130 making constitutively IL-6 receptor negative cells responsive for IL-6. The generation as well as the functional role of soluble IL-6 receptor is poorly understood. Earlier, we found that the sIL-6R levels in sera of patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) were higher than those of the control group measured by ELISA sandwich technology. In the present study we detected different levels of sIL-6R in the supernatants of lymphocyte cultures of healthy persons and patients with RA as well as SLE. Moreover, we found, that in vitro dexamethasone treatment stimulated generation of sIL-6R in both healthy persons and in active SLE, while it strongly suppressed production of sIL-6R in both RA groups. At mRNA level, we found that in SLE both the IL-6R mRNA encoding the membrane spanning and alternatively spliced (soluble) variants increased. Surprisingly, the strong decrease of sIL6R protein in RA was not found at mRNA level.

KW - Dexamethasone

KW - Rheumatoid arthritis (RA)

KW - Soluble interleukin-6 receptor (sIL-6R)

KW - Systemic lupus erythematosus (SLE)

UR - http://www.scopus.com/inward/record.url?scp=0036024920&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036024920&partnerID=8YFLogxK

U2 - 10.1556/ABiol.53.2002.3.7

DO - 10.1556/ABiol.53.2002.3.7

M3 - Article

C2 - 12371610

AN - SCOPUS:0036024920

VL - 53

SP - 307

EP - 315

JO - Acta Biologica Hungarica

JF - Acta Biologica Hungarica

SN - 0236-5383

IS - 3

ER -