A switchable light-input, light-output system modelled and constructed in yeast

Oxana Sorokina, Anita Kapus, Kata Terecskei, Laura E. Dixon, L. Kozma-Bognár, Ferenc Nagy, Andrew J. Millar

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background: Advances in synthetic biology will require spatio-temporal regulation of biological processes in heterologous host cells. We develop a light-switchable, two-hybrid interaction in yeast, based upon the Arabidopsis proteins PHYTOCHROME A and FAR-RED ELONGATED HYPOCOTYL 1-LIKE. Light input to this regulatory module allows dynamic control of a light-emitting LUCIFERASE reporter gene, which we detect by real-time imaging of yeast colonies on solid media.Results: The reversible activation of the phytochrome by red light, and its inactivation by far-red light, is retained. We use this quantitative readout to construct a mathematical model that matches the system's behaviour and predicts the molecular targets for future manipulation.Conclusion: Our model, methods and materials together constitute a novel system for a eukaryotic host with the potential to convert a dynamic pattern of light input into a predictable gene expression response. This system could be applied for the regulation of genetic networks - both known and synthetic.

Original languageEnglish
Article number15
JournalJournal of Biological Engineering
Volume3
DOIs
Publication statusPublished - Sep 17 2009

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Yeast
Yeasts
Light
Arabidopsis Proteins
Synthetic Biology
Phytochrome
Biological Phenomena
Hypocotyl
Reporter Genes
Gene expression
Theoretical Models
Genes
Chemical activation
Mathematical models
Proteins
Gene Expression
Imaging techniques

ASJC Scopus subject areas

  • Biomedical Engineering
  • Environmental Engineering
  • Cell Biology
  • Molecular Biology

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A switchable light-input, light-output system modelled and constructed in yeast. / Sorokina, Oxana; Kapus, Anita; Terecskei, Kata; Dixon, Laura E.; Kozma-Bognár, L.; Nagy, Ferenc; Millar, Andrew J.

In: Journal of Biological Engineering, Vol. 3, 15, 17.09.2009.

Research output: Contribution to journalArticle

Sorokina, Oxana ; Kapus, Anita ; Terecskei, Kata ; Dixon, Laura E. ; Kozma-Bognár, L. ; Nagy, Ferenc ; Millar, Andrew J. / A switchable light-input, light-output system modelled and constructed in yeast. In: Journal of Biological Engineering. 2009 ; Vol. 3.
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AU - Nagy, Ferenc

AU - Millar, Andrew J.

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