Thirty-two specimens of 11 freshwater fish species from Lake Biwa, Japan were surveyed for coccidian infections. Four fish species proved to be infected with apicomplexans belonging to the genus Goussia. Altogether, seven Goussia species were found. Oocysts of four species inhabiting the intestinal epithelium were shed at the sporulated stage. Two other intestinal species left the fish unsporulated. A few relatively large and aged oocysts of a further species were found in the renal tubules. The sporulated intestinal species were described as Goussia biwaensis n. sp., G. grygieri n. sp., G. nipponica n. sp. and G. wakabayashii n. sp.
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