A study on the culture medium antigens of Cystcercus cellulosae for detecting antibodies of cysticercosis by means of ABC-ELISA

H. Guo, Z. Zhao, D. Z. Shi

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Abstract

Two antigens of Cysticercus cellulosae ,cystic fluid antigen (CFA) and the culture medium antigen (CMA), were used in Avidin - Biotin Peroxdase Complex - ELISA (ABC - ELISA) to detect IgG antibodies in 45 cases of cysticercosis treated with praziquantel. The results revealed the total positive rates as 51.11% with CMA and 82.22% with CFA. The positive rates in the cases treated within 2 courses of treatment were 79.17% for CMA and 87.50% for CFA, and only 19.05% for CMA and 71.43% for CFA in the cases treated for more then 3 courses. The fact that the positive rates decreased as the courses of treatment incerased showed that the sensitivity of CMA migth be related to the vital conditions of the worms in the body, whether alive or dead. It is, therefore, recommended that CMA has the potential to be employed in ABC - ELISA both as an indicator for diagnosing cysticercosis and as a reference for the evaluation of the treatment.

Original languageEnglish
Pages (from-to)125-127
Number of pages3
JournalSoutheast Asian Journal of Tropical Medicine and Public Health
Volume28 SUPPL. 1
Publication statusPublished - 1997

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Cysticercosis
Culture Media
Enzyme-Linked Immunosorbent Assay
Antigens
Antibodies
Avidin
Biotin
Cysticercus
Praziquantel
Therapeutics
Immunoglobulin G

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "A study on the culture medium antigens of Cystcercus cellulosae for detecting antibodies of cysticercosis by means of ABC-ELISA",
abstract = "Two antigens of Cysticercus cellulosae ,cystic fluid antigen (CFA) and the culture medium antigen (CMA), were used in Avidin - Biotin Peroxdase Complex - ELISA (ABC - ELISA) to detect IgG antibodies in 45 cases of cysticercosis treated with praziquantel. The results revealed the total positive rates as 51.11{\%} with CMA and 82.22{\%} with CFA. The positive rates in the cases treated within 2 courses of treatment were 79.17{\%} for CMA and 87.50{\%} for CFA, and only 19.05{\%} for CMA and 71.43{\%} for CFA in the cases treated for more then 3 courses. The fact that the positive rates decreased as the courses of treatment incerased showed that the sensitivity of CMA migth be related to the vital conditions of the worms in the body, whether alive or dead. It is, therefore, recommended that CMA has the potential to be employed in ABC - ELISA both as an indicator for diagnosing cysticercosis and as a reference for the evaluation of the treatment.",
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AU - Guo, H.

AU - Zhao, Z.

AU - Shi, D. Z.

PY - 1997

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N2 - Two antigens of Cysticercus cellulosae ,cystic fluid antigen (CFA) and the culture medium antigen (CMA), were used in Avidin - Biotin Peroxdase Complex - ELISA (ABC - ELISA) to detect IgG antibodies in 45 cases of cysticercosis treated with praziquantel. The results revealed the total positive rates as 51.11% with CMA and 82.22% with CFA. The positive rates in the cases treated within 2 courses of treatment were 79.17% for CMA and 87.50% for CFA, and only 19.05% for CMA and 71.43% for CFA in the cases treated for more then 3 courses. The fact that the positive rates decreased as the courses of treatment incerased showed that the sensitivity of CMA migth be related to the vital conditions of the worms in the body, whether alive or dead. It is, therefore, recommended that CMA has the potential to be employed in ABC - ELISA both as an indicator for diagnosing cysticercosis and as a reference for the evaluation of the treatment.

AB - Two antigens of Cysticercus cellulosae ,cystic fluid antigen (CFA) and the culture medium antigen (CMA), were used in Avidin - Biotin Peroxdase Complex - ELISA (ABC - ELISA) to detect IgG antibodies in 45 cases of cysticercosis treated with praziquantel. The results revealed the total positive rates as 51.11% with CMA and 82.22% with CFA. The positive rates in the cases treated within 2 courses of treatment were 79.17% for CMA and 87.50% for CFA, and only 19.05% for CMA and 71.43% for CFA in the cases treated for more then 3 courses. The fact that the positive rates decreased as the courses of treatment incerased showed that the sensitivity of CMA migth be related to the vital conditions of the worms in the body, whether alive or dead. It is, therefore, recommended that CMA has the potential to be employed in ABC - ELISA both as an indicator for diagnosing cysticercosis and as a reference for the evaluation of the treatment.

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