A study on immunotoxicological effects of subacute amitraz exposure in rats

L. Institóris, H. Banfi, Zs Lengyel, A. Papp, L. Nagymajtényi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The effects of amitraz, a formamidine pesticide, were investigated in four-week old outbred male Wistar rats on certain classic toxicological and haematological parameters as well as on specific immune functions. The animals were treated, per os by gavage for 28 days, in a five-day treatment two days break system, with 26.5, 21.1, 10.6 and 5.29 mg/kg/day amitraz. On the 29th day, organ weights of the thymus, heart, lung, spleen, liver, kidneys, adrenals, testicles and popliteal lymph node; WBC and RBC counts, Ht, MCV, haemoglobin; and cell content of the femoral bone marrow were determined. In two separate groups, the effects of amitraz on the PFC content of the spleen, and on the maximum level and time course of DTH reaction, were investigated. Amitraz in 26.5 mg/kg dose increased relative adrenal weight, and decreased relative liver weight, MCV value, PFC content of the spleen, and the maximum level of DTH reaction. The 21.1 mg/kg dose decreased only MCV value, while 10.6 mg/kg elevated the liver-to-brain weight ratio. Based of these findings, a NOEL dose of 5.29 mg/kg was determined for amitraz in this experimental system; while the LOEL doses were 10.6 mg/kg for the general toxicological, 21.1 mg/kg for the haematological and 26.5 mg/kg for the immune function parameters. The results show that the exposure sensitivity of these immune functions to amitraz is lower than that of some other toxicological and haematological parameters.

Original languageEnglish
Pages (from-to)441-445
Number of pages5
JournalHuman and Experimental Toxicology
Volume26
Issue number5
DOIs
Publication statusPublished - May 2007

Fingerprint

Rats
Liver
Toxicology
Spleen
Weights and Measures
Thymus
Organ Size
Thigh
Pesticides
Thymus Gland
amitraz
Testis
Wistar Rats
Brain
Bone
Hemoglobins
Animals
Lymph Nodes
Bone Marrow
Kidney

Keywords

  • Amitraz
  • Immunotoxicity
  • Rat

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

A study on immunotoxicological effects of subacute amitraz exposure in rats. / Institóris, L.; Banfi, H.; Lengyel, Zs; Papp, A.; Nagymajtényi, L.

In: Human and Experimental Toxicology, Vol. 26, No. 5, 05.2007, p. 441-445.

Research output: Contribution to journalArticle

@article{4c1fe06d3ba042239a798afc7568a358,
title = "A study on immunotoxicological effects of subacute amitraz exposure in rats",
abstract = "The effects of amitraz, a formamidine pesticide, were investigated in four-week old outbred male Wistar rats on certain classic toxicological and haematological parameters as well as on specific immune functions. The animals were treated, per os by gavage for 28 days, in a five-day treatment two days break system, with 26.5, 21.1, 10.6 and 5.29 mg/kg/day amitraz. On the 29th day, organ weights of the thymus, heart, lung, spleen, liver, kidneys, adrenals, testicles and popliteal lymph node; WBC and RBC counts, Ht, MCV, haemoglobin; and cell content of the femoral bone marrow were determined. In two separate groups, the effects of amitraz on the PFC content of the spleen, and on the maximum level and time course of DTH reaction, were investigated. Amitraz in 26.5 mg/kg dose increased relative adrenal weight, and decreased relative liver weight, MCV value, PFC content of the spleen, and the maximum level of DTH reaction. The 21.1 mg/kg dose decreased only MCV value, while 10.6 mg/kg elevated the liver-to-brain weight ratio. Based of these findings, a NOEL dose of 5.29 mg/kg was determined for amitraz in this experimental system; while the LOEL doses were 10.6 mg/kg for the general toxicological, 21.1 mg/kg for the haematological and 26.5 mg/kg for the immune function parameters. The results show that the exposure sensitivity of these immune functions to amitraz is lower than that of some other toxicological and haematological parameters.",
keywords = "Amitraz, Immunotoxicity, Rat",
author = "L. Instit{\'o}ris and H. Banfi and Zs Lengyel and A. Papp and L. Nagymajt{\'e}nyi",
year = "2007",
month = "5",
doi = "10.1177/0960327107076887",
language = "English",
volume = "26",
pages = "441--445",
journal = "Human and Experimental Toxicology",
issn = "0960-3271",
publisher = "SAGE Publications Inc.",
number = "5",

}

TY - JOUR

T1 - A study on immunotoxicological effects of subacute amitraz exposure in rats

AU - Institóris, L.

AU - Banfi, H.

AU - Lengyel, Zs

AU - Papp, A.

AU - Nagymajtényi, L.

PY - 2007/5

Y1 - 2007/5

N2 - The effects of amitraz, a formamidine pesticide, were investigated in four-week old outbred male Wistar rats on certain classic toxicological and haematological parameters as well as on specific immune functions. The animals were treated, per os by gavage for 28 days, in a five-day treatment two days break system, with 26.5, 21.1, 10.6 and 5.29 mg/kg/day amitraz. On the 29th day, organ weights of the thymus, heart, lung, spleen, liver, kidneys, adrenals, testicles and popliteal lymph node; WBC and RBC counts, Ht, MCV, haemoglobin; and cell content of the femoral bone marrow were determined. In two separate groups, the effects of amitraz on the PFC content of the spleen, and on the maximum level and time course of DTH reaction, were investigated. Amitraz in 26.5 mg/kg dose increased relative adrenal weight, and decreased relative liver weight, MCV value, PFC content of the spleen, and the maximum level of DTH reaction. The 21.1 mg/kg dose decreased only MCV value, while 10.6 mg/kg elevated the liver-to-brain weight ratio. Based of these findings, a NOEL dose of 5.29 mg/kg was determined for amitraz in this experimental system; while the LOEL doses were 10.6 mg/kg for the general toxicological, 21.1 mg/kg for the haematological and 26.5 mg/kg for the immune function parameters. The results show that the exposure sensitivity of these immune functions to amitraz is lower than that of some other toxicological and haematological parameters.

AB - The effects of amitraz, a formamidine pesticide, were investigated in four-week old outbred male Wistar rats on certain classic toxicological and haematological parameters as well as on specific immune functions. The animals were treated, per os by gavage for 28 days, in a five-day treatment two days break system, with 26.5, 21.1, 10.6 and 5.29 mg/kg/day amitraz. On the 29th day, organ weights of the thymus, heart, lung, spleen, liver, kidneys, adrenals, testicles and popliteal lymph node; WBC and RBC counts, Ht, MCV, haemoglobin; and cell content of the femoral bone marrow were determined. In two separate groups, the effects of amitraz on the PFC content of the spleen, and on the maximum level and time course of DTH reaction, were investigated. Amitraz in 26.5 mg/kg dose increased relative adrenal weight, and decreased relative liver weight, MCV value, PFC content of the spleen, and the maximum level of DTH reaction. The 21.1 mg/kg dose decreased only MCV value, while 10.6 mg/kg elevated the liver-to-brain weight ratio. Based of these findings, a NOEL dose of 5.29 mg/kg was determined for amitraz in this experimental system; while the LOEL doses were 10.6 mg/kg for the general toxicological, 21.1 mg/kg for the haematological and 26.5 mg/kg for the immune function parameters. The results show that the exposure sensitivity of these immune functions to amitraz is lower than that of some other toxicological and haematological parameters.

KW - Amitraz

KW - Immunotoxicity

KW - Rat

UR - http://www.scopus.com/inward/record.url?scp=34447303061&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447303061&partnerID=8YFLogxK

U2 - 10.1177/0960327107076887

DO - 10.1177/0960327107076887

M3 - Article

C2 - 17623769

AN - SCOPUS:34447303061

VL - 26

SP - 441

EP - 445

JO - Human and Experimental Toxicology

JF - Human and Experimental Toxicology

SN - 0960-3271

IS - 5

ER -