A study of the effect of proteins and endogenous cations on a lipophilic β-cyclodextrin-based potentiometric lidocaine sensor using discrete solution and flow-injection analysis

Ritu Kataky, K. Tóth, Simon Palmer, Zsofia Feher

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

2,6 Didodecyl β cyclodextrin (2,6ddβCD) modified ion-selective electrodes (ISEs) were used in discrete solutions and in a flow injection analysis manifold for monitoring the local anaesthetic lidocaine hydrochloride (lignocaine) in the presence of endogenous cations and proteins. Membrane matrices comprised of either high molecular weight poly (vinyl chloride) (PVC) or polyurethane (Tecoflex SG 80) were compared. The behaviour of these electrodes in the presence of bovine serum albumin (BSA), α1 acid glycoprotein (AAG) and human serum (HS) indicate that the Tecoflex- based membrane matrices are preferable to PVC as the drift induced by proteins on baseline lines and peak height reproducibility was considerably reduced in the former. Interference from 'serum' levels of sodium, potassium and calcium (145 mmol dm-3 Na+, 1.26 mmol dm-3 Ca2+, 4.30 mmol dm-3 K+) was negligible (-Log K(ij)/(POI) (overall) ≥ 4.0). The major organic interferents were molecules of similar size and structure, which caused reduced slope and drift in baseline potentials, at equimolar concentration levels. A reduction in interferent concentration by a factor of 10 negated these effects.

Original languageEnglish
Pages (from-to)939-946
Number of pages8
JournalTalanta
Volume50
Issue number5
DOIs
Publication statusPublished - Dec 1999

Fingerprint

Potentiometric sensors
Flow Injection Analysis
Cyclodextrins
Lidocaine
Polyvinyl Chloride
Cations
Membranes
Vinyl Chloride
Ion-Selective Electrodes
Polyurethanes
Bovine Serum Albumin
Local Anesthetics
Serum
Glycoproteins
Potassium
Electrodes
Proteins
Molecular Weight
Sodium
Molecular weight

Keywords

  • Cyclodextrins
  • Ion-selective electrodes
  • Lidocaine
  • Proteins

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

A study of the effect of proteins and endogenous cations on a lipophilic β-cyclodextrin-based potentiometric lidocaine sensor using discrete solution and flow-injection analysis. / Kataky, Ritu; Tóth, K.; Palmer, Simon; Feher, Zsofia.

In: Talanta, Vol. 50, No. 5, 12.1999, p. 939-946.

Research output: Contribution to journalArticle

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