A splice donor site mutation results in the insertion of five extra amino acids into P53 from SEWA mouse sarcoma cells

Kristinn P. Magnusson, J. Mináróvits, George Klein, Klas G. Wiman

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The status of the p53 gene in SEWA-AS12-ADH (S-ADH) cells, a subline of the mouse sarcoma cell line SEWA, was examined. Immunoprecipitation with wild-type (wt) or mutant P53-specific monoclonal antibodies (mAb) showed that both wt and mutant P53 were produced. Sequence analysis of the p53 cDNA and genomic DNA revealed a single nucleotide (nt) substitution at a splice donor site at the beginning of intron 7. As a result of this mutation, an alternative splice site 15 nt further 3′ in intron 7 is used. The P53 protein translated from this aberrantly spliced mRNA carries an Arg258→Ser substitution, followed by an insertion of 5 extra amino acids. This is the first example of a splice-site mutation in the mouse p53 gene.

Original languageEnglish
Pages (from-to)231-234
Number of pages4
JournalGene
Volume162
Issue number2
Publication statusPublished - Sep 11 1995

Fingerprint

RNA Splice Sites
p53 Genes
Sarcoma
Introns
Nucleotides
Amino Acids
Mutation
Immunoprecipitation
Sequence Analysis
Complementary DNA
Monoclonal Antibodies
Cell Line
Messenger RNA
DNA
Proteins

Keywords

  • aa
  • Alternative splicing
  • amino acids (s)
  • AMV
  • avian myeloblastosis virus
  • base pair(s)
  • bp
  • gene encoding P53
  • insertion
  • mAb
  • monoclonal antibody(ies)
  • murinep53
  • nt
  • nucleotide(s)
  • p53
  • P53
  • PCR
  • polymerase chain reaction
  • S-ADH
  • SEWA-AS12-ADH mouse sarcoma cell line
  • tumor suppressor
  • tumor suppressors
  • tumorigenesis
  • wild type
  • wt

ASJC Scopus subject areas

  • Genetics

Cite this

A splice donor site mutation results in the insertion of five extra amino acids into P53 from SEWA mouse sarcoma cells. / Magnusson, Kristinn P.; Mináróvits, J.; Klein, George; Wiman, Klas G.

In: Gene, Vol. 162, No. 2, 11.09.1995, p. 231-234.

Research output: Contribution to journalArticle

Magnusson, Kristinn P. ; Mináróvits, J. ; Klein, George ; Wiman, Klas G. / A splice donor site mutation results in the insertion of five extra amino acids into P53 from SEWA mouse sarcoma cells. In: Gene. 1995 ; Vol. 162, No. 2. pp. 231-234.
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