A specific binding site for a fragment of the B-loop of epidermal growth factor and related peptides

Dmitry S. Gembitsky, Zsolt Bozsó, Martina O’Flaharty, Ferenc Ötvös, Richard F. Murphy, Sándor Lovas

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Fragments of the B-loop of the epidermal growth factor family of peptides are reported to have mitogenic and angiogenic properties but appear to fail to compete with radioiodinated EGF in receptor binding. In this study, 11 analogs of a fragment of the B-loop of EGF-related peptides from several species were synthesized to study binding to A431 human epidermoid carcinoma using both 125I-EGF and [3′4′-3H-Tyr22,29, Abu20,31]EGF(20-31)-NH2. Specific binding sites were found for the human fragment and 8 analogs at a density five times higher than that of the EGF receptors. Analogs did not compete with 125I-EGF for binding to the EGF receptor. The novel binding site may mediate the biological effects of the fragments. The primary rather than secondary structure of the fragments appears to determine affinity.

Original languageEnglish
Pages (from-to)97-102
Number of pages6
JournalPeptides
Volume23
Issue number1
DOIs
Publication statusPublished - Jan 1 2002

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Keywords

  • B-loop fragment
  • Epidermal growth factor
  • Specific binding
  • Transforming growth factor alpha

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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