A single-tube flow cytometric procedure for enhancing the diagnosis and prognostic classification of patients with myelodysplastic syndromes

B. Kárai, J. Bedekovics, Zs Miltényi, L. Gergely, L. Szerafin, A. Ujfalusi, J. Kappelmayer, Zs Hevessy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Introduction: We created a simple and effective flow cytometry scoring system (FCSS) for suspected Myelodysplastic syndromes (MDS) samples and evaluated its diagnostic and prognostic potential. Methods: Besides evaluating the four parameters suggested by Ogata, we investigated erythroid precursors and mast cells. We evaluated the six-parameter FCSS in a four-color setting (test cohort: 51 patients; 25 controls), then we implemented it into an eight-color setting and tested it on a validation cohort of patients with MDS (n=31). Results: When we compared MDS cases to non-MDS samples in the test cohort, we detected significant differences regarding not only the four major parameters but also two additional ones, namely CD71 rCV% of erythroid precursors (P=.004) and mast cell percentage (MC%) (P=.001). The utilization of the modified six-parameter FCSS provided high sensitivity and specificity both in the four color (84% and 80%, respectively) and in the eight color (81% and 100%, respectively) setting, with an excellent discriminative power between MDS and non-MDS samples. Furthermore, we found significant difference in event-free survival between the risk groups based on the modified six-parameter FCSS (P=.001). Conclusion: We evaluated and validated a single-tube flow cytometric procedure for a simple six-parameter FCSS which has not only high diagnostic but also prognostic power.

Original languageEnglish
Pages (from-to)577-584
Number of pages8
JournalInternational Journal of Laboratory Hematology
Volume39
Issue number6
DOIs
Publication statusPublished - Dec 1 2017

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Keywords

  • erythroid CD71
  • flow cytometry scoring system
  • mast cell
  • Myelodysplastic syndrome
  • rare events

ASJC Scopus subject areas

  • Hematology
  • Clinical Biochemistry
  • Biochemistry, medical

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