A single point mutation in Tomato spotted wilt virus NSs protein is sufficient to overcome Tsw-gene-mediated resistance in pepper

Asztéria Almási, Katalin Nemes, Zsófia Csömör, István Tóbiás, László Palkovics, K. Salánki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The nonstructural protein (NSs) of Tomato spotted wilt virus (TSWV) was previously identified as an avirulence determinant for Tsw-based resistance on pepper. The NSs of wild-type (WT) and resistance-breaking (RB) TSWV strains isolated in Hungary had only two amino acid substitutions (104, 461). We have analysed the ability of the NSs and their point mutant variants to trigger Tsw-mediated hypersensitive responses and RNA silencing suppressor (RSS) activity in patch assays. We identified a single amino acid change at position 104 (T-A) that was responsible for the necrosis induction or loss, while a significant difference was not detected in the RSS activity of the two parental strains. We have successfully complemented the infection of the WT strain on resistant pepper cultivar with the infectious S RNA transcript of the RB strain and the WTT104A point mutant. Our work provides direct evidence that a single amino acid change can induce an RB phenotype.

Original languageEnglish
Article number000798
Pages (from-to)1521-1525
Number of pages5
JournalJournal of General Virology
Volume98
Issue number6
DOIs
Publication statusPublished - Jun 1 2017

    Fingerprint

Keywords

  • Complementation
  • NSs
  • Resistance breaking
  • Transient expression
  • Tsw gene
  • TSWV

ASJC Scopus subject areas

  • Virology

Cite this