A simple method to assess in vivo repair of ultraviolet radiation-induced lesions of specific DNA sequences of restriction sites

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1 Citation (Scopus)

Abstract

Based on the direct relationship between ultraviolet irradiation of DNA and its susceptibility to restriction endonucleases, we have devised a simple method to quantify in vivo damage and repair of selected restriction sites. The simplicity and power of the method were demonstrated with HindIII restriction endonuclease and pAc360-501-β-gal plasmid in UV-irradiated Escherichia coli cells. The large number of available restriction endonucleases makes the method quite flexible. This method provides a simple and inexpensive means to screen mutagenic and antimutagenic drugs that interact with the DNA repair mechanism.

Original languageEnglish
Pages (from-to)397-403
Number of pages7
JournalRadiation Research
Volume136
Issue number3
Publication statusPublished - 1993

Fingerprint

ultraviolet radiation
lesions
DNA Restriction Enzymes
constrictions
restriction endonucleases
deoxyribonucleic acid
Radiation
nucleotide sequences
plasmids
Escherichia
DNA repair
methodology
DNA Repair
drugs
Plasmids
irradiation
Escherichia coli
damage
magnetic permeability
DNA

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

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abstract = "Based on the direct relationship between ultraviolet irradiation of DNA and its susceptibility to restriction endonucleases, we have devised a simple method to quantify in vivo damage and repair of selected restriction sites. The simplicity and power of the method were demonstrated with HindIII restriction endonuclease and pAc360-501-β-gal plasmid in UV-irradiated Escherichia coli cells. The large number of available restriction endonucleases makes the method quite flexible. This method provides a simple and inexpensive means to screen mutagenic and antimutagenic drugs that interact with the DNA repair mechanism.",
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AU - Kovacs, A.

AU - Gál, P.

AU - Závodszky, P.

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N2 - Based on the direct relationship between ultraviolet irradiation of DNA and its susceptibility to restriction endonucleases, we have devised a simple method to quantify in vivo damage and repair of selected restriction sites. The simplicity and power of the method were demonstrated with HindIII restriction endonuclease and pAc360-501-β-gal plasmid in UV-irradiated Escherichia coli cells. The large number of available restriction endonucleases makes the method quite flexible. This method provides a simple and inexpensive means to screen mutagenic and antimutagenic drugs that interact with the DNA repair mechanism.

AB - Based on the direct relationship between ultraviolet irradiation of DNA and its susceptibility to restriction endonucleases, we have devised a simple method to quantify in vivo damage and repair of selected restriction sites. The simplicity and power of the method were demonstrated with HindIII restriction endonuclease and pAc360-501-β-gal plasmid in UV-irradiated Escherichia coli cells. The large number of available restriction endonucleases makes the method quite flexible. This method provides a simple and inexpensive means to screen mutagenic and antimutagenic drugs that interact with the DNA repair mechanism.

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VL - 136

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