A simple method for synthesizing [γ-32P]nucleoside triphosphates using [32P]acetylphosphate and acetate kinase

Pál I. Bauer, Gyula Várady

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A simple, rapid, and inexpensive method is described for the synthesis of γ-32P-labeled ribo- or deoxyribonucleoside triphosphates. The procedure involves chemical synthesis of [32P]acetylphosphate and subsequent phosphorylation of nucleoside diphosphates using acetate kinase (EC 2.7.2.1) and a final purification step. The entire procedure is performed 8 h or less.

Original languageEnglish
Pages (from-to)613-617
Number of pages5
JournalAnalytical Biochemistry
Volume91
Issue number2
DOIs
Publication statusPublished - Dec 1978

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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