A simple method for sample preparation to facilitate efficient whole-genome sequencing of African swine fever virus

Ferenc Olasz, István Mészáros, Szilvia Marton, Gyozo L. Kaján, Vivien Tamás, Gabriella Locsmándi, Tibor Magyar, Ádám Bálint, Krisztián Bányai, Zoltán Zádori

Research output: Contribution to journalArticle

Abstract

In the recent years, African swine fever has become the biggest animal health threat to the swine industry. To facilitate quick genetic analysis of its causative agent, the African swine fever virus (ASFV), we developed a simple and efficient method for next generation sequencing of the viral DNA. Execution of the protocol does not demand complicated virus purification steps, enrichment of the virus by ultracentrifugation or of the viral DNA by ASFV-specific PCRs, and minimizes the use of Sanger sequencing. Efficient DNA-se treatment, monitoring of sample preparation by qPCR, and whole genome amplification are the key elements of the method. Through detailed description of sequencing of the first Hungarian ASFV isolate (ASFV-HU-2018), we specify the sensitive steps and supply key reference numbers to assist reproducibility and to facilitate the successful use of the method for other ASFV researchers.

Original languageEnglish
Article number1129
JournalViruses
Volume11
Issue number12
DOIs
Publication statusPublished - Dec 6 2019

    Fingerprint

Keywords

  • African swine fever virus
  • ASFV
  • DNAse treatment
  • Hungarian ASFV strain
  • Illumina
  • NGS
  • Whole genome amplification
  • Whole genome amplification
  • Whole genome sequencing

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Olasz, F., Mészáros, I., Marton, S., Kaján, G. L., Tamás, V., Locsmándi, G., Magyar, T., Bálint, Á., Bányai, K., & Zádori, Z. (2019). A simple method for sample preparation to facilitate efficient whole-genome sequencing of African swine fever virus. Viruses, 11(12), [1129]. https://doi.org/10.3390/v11121129