A simple method for isolation, liquid culture, transformation and regeneration of Arabidopsis thaliana protoplasts

Jaideep Mathur, Csaba Koncz, L. Szabados

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

An efficient technique was developed for the isolation, culture, transformation and regeneration of protoplasts derived from auxin conditioned Arabidopsis root cultures. On an average 30% of root protoplasts underwent cell division in liquid culture and formed somatic embryolike structures which regenerated to plants without embedding in Ca2+-alginate. The protoplast protocol was applicable to different landraces of Arabidopsis thaliana (L.) Heynh., such as RLD, Columbia or C24. PEG-mediated DNA uptake into protoplasts using different uidA reporter gene constructs yielded transient gene expression in over 25% of treated cells indicating that root-derived protoplasts are suitable recipients for transformation.

Original languageEnglish
Pages (from-to)221-226
Number of pages6
JournalPlant Cell Reports
Volume14
Issue number4
DOIs
Publication statusPublished - Jan 1995

Fingerprint

isolation techniques
protoplasts
Arabidopsis thaliana
liquids
gene overexpression
alginates
reporter genes
landraces
cell division
auxins
Arabidopsis
calcium
DNA
cells

Keywords

  • Arabidopsis thaliana
  • DNA transformation
  • Root derived protoplasts
  • Somatic embryogenesis
  • Transient uidA gene expression

ASJC Scopus subject areas

  • Plant Science

Cite this

A simple method for isolation, liquid culture, transformation and regeneration of Arabidopsis thaliana protoplasts. / Mathur, Jaideep; Koncz, Csaba; Szabados, L.

In: Plant Cell Reports, Vol. 14, No. 4, 01.1995, p. 221-226.

Research output: Contribution to journalArticle

@article{0263692a1607404c80cf3691e9e2de56,
title = "A simple method for isolation, liquid culture, transformation and regeneration of Arabidopsis thaliana protoplasts",
abstract = "An efficient technique was developed for the isolation, culture, transformation and regeneration of protoplasts derived from auxin conditioned Arabidopsis root cultures. On an average 30{\%} of root protoplasts underwent cell division in liquid culture and formed somatic embryolike structures which regenerated to plants without embedding in Ca2+-alginate. The protoplast protocol was applicable to different landraces of Arabidopsis thaliana (L.) Heynh., such as RLD, Columbia or C24. PEG-mediated DNA uptake into protoplasts using different uidA reporter gene constructs yielded transient gene expression in over 25{\%} of treated cells indicating that root-derived protoplasts are suitable recipients for transformation.",
keywords = "Arabidopsis thaliana, DNA transformation, Root derived protoplasts, Somatic embryogenesis, Transient uidA gene expression",
author = "Jaideep Mathur and Csaba Koncz and L. Szabados",
year = "1995",
month = "1",
doi = "10.1007/BF00233637",
language = "English",
volume = "14",
pages = "221--226",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - A simple method for isolation, liquid culture, transformation and regeneration of Arabidopsis thaliana protoplasts

AU - Mathur, Jaideep

AU - Koncz, Csaba

AU - Szabados, L.

PY - 1995/1

Y1 - 1995/1

N2 - An efficient technique was developed for the isolation, culture, transformation and regeneration of protoplasts derived from auxin conditioned Arabidopsis root cultures. On an average 30% of root protoplasts underwent cell division in liquid culture and formed somatic embryolike structures which regenerated to plants without embedding in Ca2+-alginate. The protoplast protocol was applicable to different landraces of Arabidopsis thaliana (L.) Heynh., such as RLD, Columbia or C24. PEG-mediated DNA uptake into protoplasts using different uidA reporter gene constructs yielded transient gene expression in over 25% of treated cells indicating that root-derived protoplasts are suitable recipients for transformation.

AB - An efficient technique was developed for the isolation, culture, transformation and regeneration of protoplasts derived from auxin conditioned Arabidopsis root cultures. On an average 30% of root protoplasts underwent cell division in liquid culture and formed somatic embryolike structures which regenerated to plants without embedding in Ca2+-alginate. The protoplast protocol was applicable to different landraces of Arabidopsis thaliana (L.) Heynh., such as RLD, Columbia or C24. PEG-mediated DNA uptake into protoplasts using different uidA reporter gene constructs yielded transient gene expression in over 25% of treated cells indicating that root-derived protoplasts are suitable recipients for transformation.

KW - Arabidopsis thaliana

KW - DNA transformation

KW - Root derived protoplasts

KW - Somatic embryogenesis

KW - Transient uidA gene expression

UR - http://www.scopus.com/inward/record.url?scp=0029135774&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029135774&partnerID=8YFLogxK

U2 - 10.1007/BF00233637

DO - 10.1007/BF00233637

M3 - Article

VL - 14

SP - 221

EP - 226

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 4

ER -