A simple method for isolation, liquid culture, transformation and regeneration of Arabidopsis thaliana protoplasts

Jaideep Mathur, Csaba Koncz, László Szabados

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

An efficient technique was developed for the isolation, culture, transformation and regeneration of protoplasts derived from auxin conditioned Arabidopsis root cultures. On an average 30% of root protoplasts underwent cell division in liquid culture and formed somatic embryolike structures which regenerated to plants without embedding in Ca2+-alginate. The protoplast protocol was applicable to different landraces of Arabidopsis thaliana (L.) Heynh., such as RLD, Columbia or C24. PEG-mediated DNA uptake into protoplasts using different uidA reporter gene constructs yielded transient gene expression in over 25% of treated cells indicating that root-derived protoplasts are suitable recipients for transformation.

Original languageEnglish
Pages (from-to)221-226
Number of pages6
JournalPlant Cell Reports
Volume14
Issue number4
DOIs
Publication statusPublished - Jan 1 1995

Keywords

  • Arabidopsis thaliana
  • DNA transformation
  • Root derived protoplasts
  • Somatic embryogenesis
  • Transient uidA gene expression

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Fingerprint Dive into the research topics of 'A simple method for isolation, liquid culture, transformation and regeneration of Arabidopsis thaliana protoplasts'. Together they form a unique fingerprint.

  • Cite this