A simple method for correlative light, scanning electron microscopic and X‐ray microanalytical examination of the same section

L. Siklós, M. Rózsa, J. Zombori

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3 Citations (Scopus)


Thin paraffin sections, mounted on scanning specimen holders previously coated with polyester film tape (Minnesota Mining and MFG Co., Scotch film tape No. 850 gold), were processed for light microscopy (LM) in the conventional way, then covered with celloxin shellac and examined in the LM by using the upper illuminating source. After removal of the shellac from the surface of the sample by immersion in acetone, the sections were air‐dried, coated with a copper layer in a vacuum evaporator and examined in a scanning electron microscope (SEM). The method allows: (i) high‐quality LM possibilities for establishment of the diagnosis in pathological cases; (ii) SEM examination of the same area as observed in LM; and (iii) EPMA measurements of insoluble precipitates embedded in the tissue. The usefulness of the proposed method is obvious in cases where the composition of a precipitate on LM scale is to be compared with the LM appearance of the surrounding tissue. 1986 Blackwell Science Ltd

Original languageEnglish
Pages (from-to)107-110
Number of pages4
JournalJournal of Microscopy
Issue number1
Publication statusPublished - Apr 1986



  • Light microscopy
  • X‐ray microanalysis
  • breast lesions
  • diagnostic pathology
  • microcalcifications
  • scanning electron microscopy

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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