A simple method for analysing the effects of algae on the growth of Lemna and preventing algal growth in duckweed bioassays

S. Szabó, Rudi Roijackers, Marten Scheffer

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A simple novel method for indoor culture experiments with small floating water plants, such as Lemnaceae, is described. Experiments demonstrate that the method allows for longer lasting culture experiments with Lemna, avoiding algal growth and self-shading of fronds by overcrowding. This is achieved by enclosing the Lemna fronds in tubes, which are replaced during growth by tubes of increasing diameter. The RGR of Lemna reached optimal values (RGR > 0.3 day-1) when not hampered by algal growth. In competition experiments with algae growing outside the tubes and Lemna inside the tubes, the growth of the fronds decreased dramatically; chlorophyll content of the fronds was 91-97% less and RGR 29-55% less. This method allows for multifactorial experiments, handling up to 100 experimental units per researcher. This paves the road to competition experiments among and between water plants for light, nutrients and space.

Original languageEnglish
Pages (from-to)567-575
Number of pages9
JournalArchiv fur Hydrobiologie
Volume157
Issue number4
DOIs
Publication statusPublished - 2003

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algae
Lemna
fronds
bioassay
bioassays
alga
experiment
Araceae
methodology
overcrowding
roads
shade
researchers
water
shading
chlorophyll
effect
method
nutrients
road

Keywords

  • Alga
  • Bioassay
  • Competition
  • Duckweed
  • Lemna test

ASJC Scopus subject areas

  • Aquatic Science

Cite this

A simple method for analysing the effects of algae on the growth of Lemna and preventing algal growth in duckweed bioassays. / Szabó, S.; Roijackers, Rudi; Scheffer, Marten.

In: Archiv fur Hydrobiologie, Vol. 157, No. 4, 2003, p. 567-575.

Research output: Contribution to journalArticle

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