A simple chromatographic route for the isolation of meso diaminopimelic acid

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Meso diaminopimelic acid is an important noncoded amino acid found in Gram-negative bacterial peptidoglycan. In spite of its importance, this stereoisomer is not available commercially. A simple, economical procedure was developed for the isolation of pure meso diaminopimelic acid via an high-performance liquid chromatography separation. In our new approach, the underivatized three isomers of diaminopimelic acid were separated on a crown ether-based chiral stationary phase. For the structure identification, 1H NMR spectroscopy was applied.

Original languageEnglish
Pages (from-to)133-137
Number of pages5
JournalChirality
Volume23
Issue number2
DOIs
Publication statusPublished - Feb 2011

Fingerprint

Diaminopimelic Acid
Acids
Crown Ethers
Crown ethers
Stereoisomerism
Peptidoglycan
High performance liquid chromatography
Isomers
Nuclear magnetic resonance spectroscopy
Amino acids
Magnetic Resonance Spectroscopy
High Pressure Liquid Chromatography
Amino Acids

Keywords

  • (+)-(18-crown-6)-2,3, 11,12-tetracarboxylic acid-based stationary phase
  • chiral chromatography
  • HPLC
  • meso diaminopimelic acid
  • NMR structure

ASJC Scopus subject areas

  • Organic Chemistry
  • Analytical Chemistry
  • Drug Discovery
  • Pharmacology
  • Catalysis
  • Spectroscopy

Cite this

A simple chromatographic route for the isolation of meso diaminopimelic acid. / Tóth, G.; Hetényi, A.; Ilisz, I.; Péter, A.

In: Chirality, Vol. 23, No. 2, 02.2011, p. 133-137.

Research output: Contribution to journalArticle

@article{84201af332614243a5d2ae382a6c4e1f,
title = "A simple chromatographic route for the isolation of meso diaminopimelic acid",
abstract = "Meso diaminopimelic acid is an important noncoded amino acid found in Gram-negative bacterial peptidoglycan. In spite of its importance, this stereoisomer is not available commercially. A simple, economical procedure was developed for the isolation of pure meso diaminopimelic acid via an high-performance liquid chromatography separation. In our new approach, the underivatized three isomers of diaminopimelic acid were separated on a crown ether-based chiral stationary phase. For the structure identification, 1H NMR spectroscopy was applied.",
keywords = "(+)-(18-crown-6)-2,3, 11,12-tetracarboxylic acid-based stationary phase, chiral chromatography, HPLC, meso diaminopimelic acid, NMR structure",
author = "G. T{\'o}th and A. Het{\'e}nyi and I. Ilisz and A. P{\'e}ter",
year = "2011",
month = "2",
doi = "10.1002/chir.20888",
language = "English",
volume = "23",
pages = "133--137",
journal = "Chirality",
issn = "0899-0042",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - A simple chromatographic route for the isolation of meso diaminopimelic acid

AU - Tóth, G.

AU - Hetényi, A.

AU - Ilisz, I.

AU - Péter, A.

PY - 2011/2

Y1 - 2011/2

N2 - Meso diaminopimelic acid is an important noncoded amino acid found in Gram-negative bacterial peptidoglycan. In spite of its importance, this stereoisomer is not available commercially. A simple, economical procedure was developed for the isolation of pure meso diaminopimelic acid via an high-performance liquid chromatography separation. In our new approach, the underivatized three isomers of diaminopimelic acid were separated on a crown ether-based chiral stationary phase. For the structure identification, 1H NMR spectroscopy was applied.

AB - Meso diaminopimelic acid is an important noncoded amino acid found in Gram-negative bacterial peptidoglycan. In spite of its importance, this stereoisomer is not available commercially. A simple, economical procedure was developed for the isolation of pure meso diaminopimelic acid via an high-performance liquid chromatography separation. In our new approach, the underivatized three isomers of diaminopimelic acid were separated on a crown ether-based chiral stationary phase. For the structure identification, 1H NMR spectroscopy was applied.

KW - (+)-(18-crown-6)-2,3, 11,12-tetracarboxylic acid-based stationary phase

KW - chiral chromatography

KW - HPLC

KW - meso diaminopimelic acid

KW - NMR structure

UR - http://www.scopus.com/inward/record.url?scp=78650946779&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650946779&partnerID=8YFLogxK

U2 - 10.1002/chir.20888

DO - 10.1002/chir.20888

M3 - Article

VL - 23

SP - 133

EP - 137

JO - Chirality

JF - Chirality

SN - 0899-0042

IS - 2

ER -