A simple and sensitive method of acetylcholine identification and assay. Bioassay combined with minicolumn gel filtration or high-performance liquid chromatography

E. Vízi, László G. Hársinc, Deryck Duncalf, Hideo Nagashima, Pamela Potter, Francis F. Foldes

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A modified technique of acetylcholine assay on the guinea pig ileum has been combined with either minivolume gel filtration or high-performance liquid chromatography separation of the samples. In addition, labeled acetylcholine (14C-ACh) was eluted with unlabeled acetylcholine or with the samples expected to contain acetylcholine, and their elution profiles were compared by bioassay plus radioassay of eluate fractions. When the elution of both biological activity and label occurred in the same eluates, it was concluded that the substance assayed on guinea pig ileum was acetylcholine. The method was sensitive to 0.5 ng (1.8 pmol) of acetylcholine and its reproducibility was within 5%. It thus represents a substantial improvement in chemical specificity over the previous bioassay method described by Paton and Vizi (1969).

Original languageEnglish
Pages (from-to)201-211
Number of pages11
JournalJournal of Pharmacological Methods
Volume13
Issue number3
DOIs
Publication statusPublished - 1985

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Bioassay
High performance liquid chromatography
Biological Assay
Acetylcholine
Gel Chromatography
Assays
Gels
High Pressure Liquid Chromatography
Ileum
Guinea Pigs
Bioactivity
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ASJC Scopus subject areas

  • Pharmacology

Cite this

A simple and sensitive method of acetylcholine identification and assay. Bioassay combined with minicolumn gel filtration or high-performance liquid chromatography. / Vízi, E.; Hársinc, László G.; Duncalf, Deryck; Nagashima, Hideo; Potter, Pamela; Foldes, Francis F.

In: Journal of Pharmacological Methods, Vol. 13, No. 3, 1985, p. 201-211.

Research output: Contribution to journalArticle

Vízi, E. ; Hársinc, László G. ; Duncalf, Deryck ; Nagashima, Hideo ; Potter, Pamela ; Foldes, Francis F. / A simple and sensitive method of acetylcholine identification and assay. Bioassay combined with minicolumn gel filtration or high-performance liquid chromatography. In: Journal of Pharmacological Methods. 1985 ; Vol. 13, No. 3. pp. 201-211.
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AU - Foldes, Francis F.

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