A simple and rapid method enhancing of lipoxygenase-1 to lipoxygenase2+lipoxygenase-3 isoenzyme activity ratio in soybean meal extracts

A. S. Nemeth, B. Szajáni, J. S. Marczy, M. L. Simon

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Lipoxygenase-2 and lipoxygenase-3 isoenzymes can be eliminated from soybean (Glycine max) meal extract by a simple selective heat treatment. The optimum conditions were: 70 °C, for 5 min at pH 5.2 with an ionic strength of 0.05. The activity ratio of lipoxygenase-1 to lipoxygenase-2+lipoxygenase-3 was enhanced from 5 to about 21. The resulting enzyme can be used immediately for hydroperoxidation or frozen without loss of activity.

Original languageEnglish
Pages (from-to)389-392
Number of pages4
JournalBiotechnology Techniques
Volume12
Issue number5
DOIs
Publication statusPublished - 1998

Fingerprint

Isoenzymes
Lipoxygenase
Ionic strength
Soybeans
Meals
Amino acids
Enzymes
Heat treatment
Osmolar Concentration
Glycine
Hot Temperature
lipoxygenase 3

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

A simple and rapid method enhancing of lipoxygenase-1 to lipoxygenase2+lipoxygenase-3 isoenzyme activity ratio in soybean meal extracts. / Nemeth, A. S.; Szajáni, B.; Marczy, J. S.; Simon, M. L.

In: Biotechnology Techniques, Vol. 12, No. 5, 1998, p. 389-392.

Research output: Contribution to journalArticle

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