A novel, time- and; labour-saving protocol for the genetic transformation and regeneration of eggplant (Solatium melongena L.) is described. In this method, co- cultivation with Agrobacterium, callus regeneration and organogenesis are carried out in one step via the decapitation of rooted hypocotyls. Plantlets expressing the Nptll gene as a selectable marker and the cucumber mosaic cucumovirus coat, protein transgene were regenerated on TMR medium containing 100 mg/1 kanamycin. The structural integrity and expression of the introduced viral gene were investigated and verified by PCR and Northern hybridization, respectively.
|Number of pages||7|
|Journal||Acta Agronomica Hungarica|
|Publication status||Published - Dec 1 1998|
- Cucumber mosaic cucumovirus (CMV)
- Plant transformation
ASJC Scopus subject areas
- Agronomy and Crop Science