A simple and efficient method for the transformation of eggplant

A. Szász, D. Szilassy, K. Salánki, M. FÁri, E. Balázs

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A novel, time- and; labour-saving protocol for the genetic transformation and regeneration of eggplant (Solatium melongena L.) is described. In this method, co- cultivation with Agrobacterium, callus regeneration and organogenesis are carried out in one step via the decapitation of rooted hypocotyls. Plantlets expressing the Nptll gene as a selectable marker and the cucumber mosaic cucumovirus coat, protein transgene were regenerated on TMR medium containing 100 mg/1 kanamycin. The structural integrity and expression of the introduced viral gene were investigated and verified by PCR and Northern hybridization, respectively.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalActa Agronomica Hungarica
Volume46
Issue number3
Publication statusPublished - 1998

Fingerprint

eggplants
decapitation
Cucumber mosaic virus
genetic transformation
kanamycin
coat proteins
organogenesis
Agrobacterium
Northern blotting
hypocotyls
transgenes
plantlets
labor
callus
genes
methodology

Keywords

  • Cucumber mosaic cucumovirus (CMV)
  • Eggplant
  • Plant transformation

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

A simple and efficient method for the transformation of eggplant. / Szász, A.; Szilassy, D.; Salánki, K.; FÁri, M.; Balázs, E.

In: Acta Agronomica Hungarica, Vol. 46, No. 3, 1998, p. 201-207.

Research output: Contribution to journalArticle

Szász, A. ; Szilassy, D. ; Salánki, K. ; FÁri, M. ; Balázs, E. / A simple and efficient method for the transformation of eggplant. In: Acta Agronomica Hungarica. 1998 ; Vol. 46, No. 3. pp. 201-207.
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