A simple and efficient method for the transformation of eggplant

A. Szász, D. Szilassy, K. Salánki, M. FÁri, E. Balazs

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A novel, time- and; labour-saving protocol for the genetic transformation and regeneration of eggplant (Solatium melongena L.) is described. In this method, co- cultivation with Agrobacterium, callus regeneration and organogenesis are carried out in one step via the decapitation of rooted hypocotyls. Plantlets expressing the Nptll gene as a selectable marker and the cucumber mosaic cucumovirus coat, protein transgene were regenerated on TMR medium containing 100 mg/1 kanamycin. The structural integrity and expression of the introduced viral gene were investigated and verified by PCR and Northern hybridization, respectively.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalActa Agronomica Hungarica
Volume46
Issue number3
Publication statusPublished - Dec 1 1998

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Keywords

  • Cucumber mosaic cucumovirus (CMV)
  • Eggplant
  • Plant transformation

ASJC Scopus subject areas

  • Agronomy and Crop Science

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