A short histone H3 promoter from alfalfa specifies expression in s-phase cells and meristems

Tamás Kapros, Iván Stefanov, Zoltán Magyar, Imre Ocsovszky, Dénes Dudits

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Cell division-dependent activity of a histone H3 promoter from alfalfa (Medicago sativa L.) has been shown through the analysis of both transgenic Nicotiana tabacum L. plants and a M. varia L. cell line. A chimeric gene was used to assay the activity of the histone H3 promoter in both transgenic systems. The chimeric gene was composed of 284 bp of 5′ upstream sequence from an alfalfa histone H3 genomic clone fused to the β-glucuronidase coding region and nopaline synthase terminator. The 284 bp of histone H3 promoter sequence specified histochemically detectable β-glucuronidase expression in the peripheral zone of the shoot apical meristem and the root tip of transgenic tobacco plants. High β-glucuronidase activity was as observed in young leaf and callus tissues. In protoplast-derived cells, an increased histone H3 promoter function was detected in 4- to 5-day old cultures and correlated with elevated incorporation of [3H]thymidine. Northern blot analysis of RNA samples from synchronized cell suspension cultures has revealed maximum accumulation of both β-glucuronidase and endogenous histone H3 mRNAs in cells at S phase. Whereas the abundance of endogenous histone H3 transcripts declined abruptly after S-phase, the reporter gene showed a more gradual decrease in transcript level after the reduction in the number of S-phase cells. The possible role of the short histone H3 promoter sequence in the regulation of S-phase-dependent expression and the involvement of the 3′ end region of histone H3 genes in mRNA stability will be discussed.

Original languageEnglish
Pages (from-to)27-32
Number of pages6
JournalIn Vitro Cellular & Developmental Biology - Plant
Volume29
Issue number1
DOIs
Publication statusPublished - Jan 1 1993

Keywords

  • alfalfa
  • cell cycle
  • cell synchronization
  • histone H3
  • tobacco
  • β-glucuronidase

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

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