A serine-proteinase inhibitor (Boc-D-Phe-Pro-Arg-H) inhibits the secretion of adrenocorticotropin- and β-endorphin-immunoreactive peptides in vitro

I. Barna, L. Gráf, G. Makara, Gyorgy Rappay

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The effects of a potent serine protease inhibitor, Boc-D-Phe-Pro-Arg-H, on the secretion and content of ACTH and β-endorphin-like immunoreactivities of cultured rat anterior pituitary cells were studied. Basal release of the hormones was not affected by the drug but secretion in response to stalkmedian eminence extracts was inhibited. The inhibitor did not effect the amount of ACTH or β-endorphin in the medium plus the cells, nor did it change the ratio of the lipotropin and β-endorphin-like immunoreactivities. Thus the compound seems to affect the release of ACTH and β-endorphin, but not the processing of lipotropin to β-endorphin.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalNeuropeptides
Volume3
Issue number1
DOIs
Publication statusPublished - 1982

Fingerprint

phenylalanyl-prolyl-arginine
Endorphins
Serine Proteinase Inhibitors
Adrenocorticotropic Hormone
Peptides
beta-Lipotropin
Rats
butyloxycarbonyl-phenylalanyl-prolyl-arginine
In Vitro Techniques
Hormones
Processing
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Clinical Neurology
  • Neuroscience(all)
  • Cellular and Molecular Neuroscience

Cite this

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abstract = "The effects of a potent serine protease inhibitor, Boc-D-Phe-Pro-Arg-H, on the secretion and content of ACTH and β-endorphin-like immunoreactivities of cultured rat anterior pituitary cells were studied. Basal release of the hormones was not affected by the drug but secretion in response to stalkmedian eminence extracts was inhibited. The inhibitor did not effect the amount of ACTH or β-endorphin in the medium plus the cells, nor did it change the ratio of the lipotropin and β-endorphin-like immunoreactivities. Thus the compound seems to affect the release of ACTH and β-endorphin, but not the processing of lipotropin to β-endorphin.",
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T1 - A serine-proteinase inhibitor (Boc-D-Phe-Pro-Arg-H) inhibits the secretion of adrenocorticotropin- and β-endorphin-immunoreactive peptides in vitro

AU - Barna, I.

AU - Gráf, L.

AU - Makara, G.

AU - Rappay, Gyorgy

PY - 1982

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N2 - The effects of a potent serine protease inhibitor, Boc-D-Phe-Pro-Arg-H, on the secretion and content of ACTH and β-endorphin-like immunoreactivities of cultured rat anterior pituitary cells were studied. Basal release of the hormones was not affected by the drug but secretion in response to stalkmedian eminence extracts was inhibited. The inhibitor did not effect the amount of ACTH or β-endorphin in the medium plus the cells, nor did it change the ratio of the lipotropin and β-endorphin-like immunoreactivities. Thus the compound seems to affect the release of ACTH and β-endorphin, but not the processing of lipotropin to β-endorphin.

AB - The effects of a potent serine protease inhibitor, Boc-D-Phe-Pro-Arg-H, on the secretion and content of ACTH and β-endorphin-like immunoreactivities of cultured rat anterior pituitary cells were studied. Basal release of the hormones was not affected by the drug but secretion in response to stalkmedian eminence extracts was inhibited. The inhibitor did not effect the amount of ACTH or β-endorphin in the medium plus the cells, nor did it change the ratio of the lipotropin and β-endorphin-like immunoreactivities. Thus the compound seems to affect the release of ACTH and β-endorphin, but not the processing of lipotropin to β-endorphin.

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