A sensitive, validated gas-chromatographic bioanalytical method by nitrogen selective detection of deramciclane in dog plasma

I. Klebovich, J. Leibinger, M. Abermann, G. Grezal

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A validated bioanalytical method has been developed for the determination of a new anxiolytic compound of deramciclane in dog plasma, using Lichrolut RP-18 solid-phase extraction and gas chromatography with nitrogen-phosphorous selective detector (GC-NPD). Gas-chromatographic separation was carried out by SPB-5 fused silica capillary column (30 m x 0.25 mm x 0.25 μm) after split injection, when the split liner was packed (3% silicone SE 30). An optimized two-level oven temperature program was used for the separation. The assay was validated with respect to selectivity, specificity, linearity, sensitivity, accuracy, precision, system suitability and plasma stability. All validation parameters were found to be within the internationally required limits. The limit of quantification of deramciclane in dog plasma was found to be 0.5 ng mL-1. The calibration curve showed good linearity (r = 0.999) over the 0.5 and 100 ng mL-1 deramciclane plasma concentration range. The assay is sufficiently sensitive and relatively fast (12.5 min chromatographic run) to be used for routine monitoring and pharmacokinetic studies of deramciclane.

Original languageEnglish
Pages (from-to)497-501
Number of pages5
JournalPharmaceutical Sciences
Volume3
Issue number10
Publication statusPublished - 1997

Fingerprint

Nitrogen
Gases
Dogs
Silicone Elastomers
Anti-Anxiety Agents
Solid Phase Extraction
Silicones
Silicon Dioxide
Gas Chromatography
Calibration
Pharmacokinetics
Sensitivity and Specificity
Injections
Temperature
deramciclane

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

A sensitive, validated gas-chromatographic bioanalytical method by nitrogen selective detection of deramciclane in dog plasma. / Klebovich, I.; Leibinger, J.; Abermann, M.; Grezal, G.

In: Pharmaceutical Sciences, Vol. 3, No. 10, 1997, p. 497-501.

Research output: Contribution to journalArticle

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