A second groEL-like gene, organized in a groESL operon is present in the genome of Synechocystis sp. PCC 6803

Csaba Lehel, Dmitry Los, Hajime Wada, János Györgyei, I. Horváth, Eszter Kovács, Norio Murata, L. Vígh

Research output: Contribution to journalArticle

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Abstract

Using a groEL gene of Synechococcus sp. PCC 7942 as a DNA probe, a 4.8-kilobase pair (kbp) BamHI fragment of chromosomal DNA of Synechocystis sp. PCC 6803 was cloned. Sequencing of 3.25 kbp of the BamHI fragment revealed three open reading frames. The amino acid sequences deduced from the nucleotide sequences of the two open reading frames are identical to those gained from N-terminal sequencing of purified groEL and groES proteins. This finding demonstrates that these two open reading frames correspond to groEL and groES genes of Synechocystis sp. PCC 6803. groEL of Synechocystis sp. PCC 6803 is remarkably homologous to groEL proteins of other organisms. Southern blot analysis indicates that only one groESL operon is present in the genomic DNA of Synechocystis sp. PCC 6803, whereas the existence of at least two copies of groEL-analogous genes are anticipated. The level of the bicistronic, 2.2-kb transcript of groESL operon increased 100-fold within 15 min upon heat stress. A 9-base pair inverted repeat revealed around the groESL promoter might be involved in regulation of the heat shock response.

Original languageEnglish
Pages (from-to)1799-1804
Number of pages6
JournalJournal of Biological Chemistry
Volume268
Issue number3
Publication statusPublished - Jan 25 1993

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Synechocystis
Operon
Genes
Genome
Open Reading Frames
DNA
DNA Probes
Synechococcus
Heat-Shock Response
Proteins
Nucleotides
Southern Blotting
Base Pairing
Amino Acids
Amino Acid Sequence
Hot Temperature

ASJC Scopus subject areas

  • Biochemistry

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A second groEL-like gene, organized in a groESL operon is present in the genome of Synechocystis sp. PCC 6803. / Lehel, Csaba; Los, Dmitry; Wada, Hajime; Györgyei, János; Horváth, I.; Kovács, Eszter; Murata, Norio; Vígh, L.

In: Journal of Biological Chemistry, Vol. 268, No. 3, 25.01.1993, p. 1799-1804.

Research output: Contribution to journalArticle

Lehel, Csaba ; Los, Dmitry ; Wada, Hajime ; Györgyei, János ; Horváth, I. ; Kovács, Eszter ; Murata, Norio ; Vígh, L. / A second groEL-like gene, organized in a groESL operon is present in the genome of Synechocystis sp. PCC 6803. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 3. pp. 1799-1804.
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AU - Györgyei, János

AU - Horváth, I.

AU - Kovács, Eszter

AU - Murata, Norio

AU - Vígh, L.

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