A salting-out assisted liquid-liquid microextraction procedure for determination of cysteine followed by spectrophotometric detection

Alina Diuzheva, József Balogh, Yaroslav Studenyak, Zoltán Cziáky, J. Jekó́

Research output: Contribution to journalArticle

Abstract

A new analytical method for sensitive determination of cysteine based on its interaction with phenazine methosulfate was developed using salting-out liquid-liquid microextraction followed by spectrophotometric detection. The mechanism of the reaction was studied and confirmed by Fourier transform infrared and mass spectroscopy. Experimental parameters affecting the extraction efficiency were investigated and under the optimal conditions, good linearity was observed in the range 0.2 – 6.0 µg mL−1 with a correlation coefficient of 0.9972. The limit of detection and limit of quantification were found to be 0.07 and 0.21 µg mL −1, respectively. The enrichment factor was 25. The developed methodology was applied for analysis of cysteine in food supplements. The obtained data were in good agreement with LC-MS/MS analysis.

LanguageEnglish
Pages446-451
Number of pages6
JournalTalanta
Volume194
DOIs
Publication statusPublished - Mar 1 2019

Fingerprint

Liquid Phase Microextraction
Methylphenazonium Methosulfate
Spectrophotometry
Spectrophotometers
Mass spectrometry
Cysteine
Amino acids
Mass Spectrometry
Amino Acids
Liquids
Fourier Transform Infrared Spectroscopy
Dietary Supplements
Limit of Detection
Fourier transforms
Spectroscopy
Infrared radiation

Keywords

  • Cysteine
  • Phenazine methosulfate
  • Salting-out assisted liquid-liquid microextraction
  • Spectrophotometry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry(all)
  • Biochemistry
  • Spectroscopy

Cite this

A salting-out assisted liquid-liquid microextraction procedure for determination of cysteine followed by spectrophotometric detection. / Diuzheva, Alina; Balogh, József; Studenyak, Yaroslav; Cziáky, Zoltán; Jekó́, J.

In: Talanta, Vol. 194, 01.03.2019, p. 446-451.

Research output: Contribution to journalArticle

Diuzheva, Alina ; Balogh, József ; Studenyak, Yaroslav ; Cziáky, Zoltán ; Jekó́, J. / A salting-out assisted liquid-liquid microextraction procedure for determination of cysteine followed by spectrophotometric detection. In: Talanta. 2019 ; Vol. 194. pp. 446-451.
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