A Restorer of Fertility-like PPR gene is required for 5a′-end processing of the nad4 mRNA in mitochondria of arabidopsis thaliana

Angela Hölzle, Christian Jonietz, Ottó Törjek, Thomas Altmann, Stefan Binder, Joachim Forner

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Summary Processing of 5a′-ends is a frequently observed step during maturation of plant mitochondrial mRNAs. Up to now, very little is known about the biochemistry of this process and the proteins involved in the removal of 5a′ leader sequences. Based on natural genetic variation we have used linkage mapping and complementation studies to identify a nuclear gene required for the efficient generation of a 5a′-end 228 nucleotides upstream of the mitochondrial nad4 gene encoding subunit 4 of the NADH dehydrogenase complex. This nuclear gene, At1g12700, that we designate RNA PROCESSING FACTOR 1 (RPF1), encodes a pentatricopeptide repeat (PPR) protein of the P-class containing canonical PPR-repeats. RPF1 belongs to a subgroup of PPR proteins, which includes the RESTORER OF FERTILITY (RF) gene products restoring cytoplasmic male sterility (CMS) in various plant species. CMS is a mitochondrially inherited trait caused by the expression of aberrant, chimeric genes, which has not been observed in the predominantly inbreeding species Arabidopsis thaliana. The here reported results are a further step towards the characterization of the plant mitochondrial RNA processing machinery and provide additional insights into the function of RF-like PPR proteins.

Original languageEnglish
Pages (from-to)737-744
Number of pages8
JournalPlant Journal
Volume65
Issue number5
DOIs
Publication statusPublished - Mar 1 2011

    Fingerprint

Keywords

  • 5a′ processing
  • Arabidopsis thaliana
  • mitochondria
  • pentatricopeptide repeat proteins
  • restorer of fertility

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this