A reagentless amperometric electrode based on carbon paste, chemically modified with D‐lactate dehydrogenase, NAD+, and mediator containing polymer for D‐lactic acid analysis. I. Construction, composition, and characterization

Hun‐Chi ‐C Shu, Bo Mattiasson, Björn Persson, Geza Nagy, Lo Gorton, Suresh Sahni, Lin Geng, Leonid Boguslavsky, Terje Skotheim

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37 Citations (Scopus)

Abstract

A reagentless carbon paste electrode was designed for D‐lactic acid analysis in a flow injection system for the monitoring of the production of D‐lactate in a batch fermentation. D‐Lactate dehydrogenase, nicotinamide adenine dinucleotide (NAD+), a synthetic redox polymer containing covalently attached toluidine blue O as mediator, graphite powder, and paraffin oil were used for the construction of the modified carbon paste electrode. D‐Lactate selectivity was indicated by insignificant responses from a variety of possible interfernces including L‐lactate. The electrodes gave a linear response in the range between 0.05 and 5 mM D‐lactate, with a detecting limit of 30 μM, allowing a sample throughput of 20 h−1. Preliminary investigations were made by covering the electrode surface with electropolymerized membranes. Satisfactory stability was observed, indicated by a reproducibility of 3.3% relative standard deviation (RSD, n = 31), with a non‐membrane‐covered electrode for the analysis of D‐lactate in fermentation broth. A long‐term stability (230 broth samples) was proven, suggesting the electrodes to have a good potential for use in on‐line monitoring of fermentation processes. © 1995 John Wiley & Sons, Inc.

Original languageEnglish
Pages (from-to)270-279
Number of pages10
JournalBiotechnology and Bioengineering
Volume46
Issue number3
DOIs
Publication statusPublished - May 5 1995

Keywords

  • D‐lactate
  • D‐lactate dehydrogenase
  • biosensor
  • carbon paste electrode
  • redox polymer

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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