A reagentless amperometric electrode based on carbon paste, chemically modified with D-lactate dehydrogenase, NAD+, and mediator containing polymer for D-lactic acid analysis. I. Construction, composition, and characterization

H. C. Shu, B. Mattiasson, B. Persson, G. Nagy, L. Gorton, S. Sahni, L. Geng, L. Boguslavsky, T. Skotheim

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A reagentless carbon paste electrode was designed for D-lactic acid analysis in a flow injection system for the monitoring of the production of D-lactate in a batch fermentation. D-Lactate dehydrogenase, nicotinamide adenine dinucleotide (NAD+), a synthetic redox polymer containing covalently attached toluidine blue O as mediator, graphite powder, and paraffin oil were used for the construction of the modified carbon paste electrode. D-Lactate selectivity was indicated by insignificant responses from a variety of possible interferences including L-lactate. The electrodes gave a linear response in the range between 0.05 and 5 mM D-lactate, with a detecting limit of 30 μM, allowing a sample throughput of 20 h-1. Preliminary investigations were made by covering the electrode surface with electropolymerized membranes. Satisfactory stability was observed, indicated by a reproducibility of 3.3% relative standard deviation (RSD, n = 31), with a non-membrane-covered electrode for the analysis of D-lactate in fermentation broth. A long-term stability (230 broth samples) was proven, suggesting the electrodes to have a good potential for use in on-line monitoring of fermentation processes.

Original languageEnglish
Pages (from-to)270-279
Number of pages10
JournalBiotechnology and Bioengineering
Volume46
Issue number3
Publication statusPublished - May 5 1995

Fingerprint

Lactic acid
Ointments
Lactic Acid
Polymers
Electrodes
Carbon
Chemical analysis
Fermentation
NAD
Paraffin oils
Tolonium Chloride
Graphite
Monitoring
Powders
Oxidation-Reduction
D-lactate dehydrogenase
Oxidoreductases
Throughput
Membranes
Injections

Keywords

  • biosensor
  • carbon paste electrode
  • D-lactate
  • D-lactate dehydrogenase
  • redox polymer

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

A reagentless amperometric electrode based on carbon paste, chemically modified with D-lactate dehydrogenase, NAD+, and mediator containing polymer for D-lactic acid analysis. I. Construction, composition, and characterization. / Shu, H. C.; Mattiasson, B.; Persson, B.; Nagy, G.; Gorton, L.; Sahni, S.; Geng, L.; Boguslavsky, L.; Skotheim, T.

In: Biotechnology and Bioengineering, Vol. 46, No. 3, 05.05.1995, p. 270-279.

Research output: Contribution to journalArticle

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AU - Persson, B.

AU - Nagy, G.

AU - Gorton, L.

AU - Sahni, S.

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