A rapid and simple method for detection of Asn363Ser polymorphism of the human glucocorticoid receptor gene

Judit Majnik, Attila Patócs, Katalin Balogh, Miklós Tóth, Károly Rácz

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20 Citations (Scopus)


Asn363Ser polymorphism of the human glucocorticoid receptor has been detected in approximately 4% of the population and it has been associated with several diseases and pathologic conditions. Here we describe a new, simple and cost-effective allele-specific PCR method for a rapid screening of this polymorphism. When compared to currently used PCR-based restriction fragment length polymorphism (RFLP) and direct DNA sequencing methods, the new allele-specific PCR method showed 100% accuracy for the detection of Asn363Asn and Asn363Ser genotypes. The feasibility of these methods were tested in 301 patients, including 47 patients with postmenopausal osteoporosis in whom the frequency of Asn363Ser polymorphism was similar to that found in control subjects (4.3% versus 4.4%).

Original languageEnglish
Pages (from-to)465-468
Number of pages4
JournalJournal of Steroid Biochemistry and Molecular Biology
Issue number5
Publication statusPublished - Dec 1 2004



  • Allele-specific PCR
  • Genetic polymorphism
  • Glucocorticoid receptor

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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