A product of the bicistronic Drosophila melanogaster gene CG31241, which also encodes a trimethylguanosine synthase, plays a role in telomere protection

Orban Komony, Tamas Schauer, Gabor Papai, P. Deák, I. Boros

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Although telomere formation occurs through a different mechanism in Drosophila compared with other organisms, telomere associations result from mutations in homologous genes, indicating the involvement of similar pathways in chromosome end protection. We report here that mutations of the Drosophila melanogaster gene CG31241 lead to high frequency chromosome end fusions. CG31241 is a bicistronic gene that encodes trimethylguanosine synthase (TGS1), which forms the m3G caps of noncoding small RNAs, and a novel protein, DTL. We show that although TGS1 has no role in telomere protection, DTL is localized at specific sites, including the ends of polytene chromosomes, and its loss results in telomere associations. Mutations of ATM- and Rad3-related (ATR) kinase suppress telomere fusions in the absence of DTL. Thus, genetic interactions place DTL in an ATR-related pathway in telomere protection. In contrast to ATR kinase, mutations of ATM (ataxia telangiectasia mutated) kinase, which acts in a partially overlapping pathway of telomere protection, do not suppress formation of telomere associations in the absence of DTL. Thus, uncovering the role of DTL will help to dissect the evolutionary conserved pathway(s) controlling ATM-ATR-related telomere protection.

Original languageEnglish
Pages (from-to)769-774
Number of pages6
JournalJournal of Cell Science
Volume122
Issue number6
DOIs
Publication statusPublished - Mar 15 2009

Fingerprint

Telomere
Drosophila melanogaster
Genes
Ataxia Telangiectasia
Mutation
Phosphotransferases
Chromosomes
Polytene Chromosomes
trimethylguanosine synthase
Small Untranslated RNA
Drosophila

Keywords

  • Ataxia telangiectasia mutated
  • ATR
  • Bicistronic gene
  • Drosophila
  • Telomere

ASJC Scopus subject areas

  • Cell Biology

Cite this

A product of the bicistronic Drosophila melanogaster gene CG31241, which also encodes a trimethylguanosine synthase, plays a role in telomere protection. / Komony, Orban; Schauer, Tamas; Papai, Gabor; Deák, P.; Boros, I.

In: Journal of Cell Science, Vol. 122, No. 6, 15.03.2009, p. 769-774.

Research output: Contribution to journalArticle

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