A priori resolution of the intermediate spectra in the bacteriorhodopsin photocycle: The time evolution of the L spectrum revealed

L. Zimányi, Jack Saltiel, Leonid S. Brown, Janos K. Lanyi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Resolution of the spectra of the intermediates in the photocycle of wild-type bacteriorhodopsin (BR) was achieved by singular value decomposition with exponential-fit-assisted self-modeling (SVD-EFASM) treatment of multichannel difference spectra measured at 5 °C during the course of the photocycle. New is the finding that two spectrally distinct L intermediates, L 1 and L 2, form sequentially. Our conclusion is that the photocycle is more complex than most published schemes. The dissection of the spectrally different L forms eliminates stoichiometric discrepancies usually appearing as systematically varying total intermediate concentrations before the onset of BR recovery. In addition, our analysis reveals that the red tails in the spectra of K and L 1 are more substantial than those of L 2 and BR. We suggest that these subtle differences in the shapes of the spectra reflect torsional and/or environmental differences in the retinyl chromophore.

Original languageEnglish
Pages (from-to)2318-2321
Number of pages4
JournalJournal of Physical Chemistry A
Volume110
Issue number7
DOIs
Publication statusPublished - Feb 23 2006

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Bacteriorhodopsins
Dissection
Singular value decomposition
Chromophores
dissection
chromophores
Recovery
recovery
decomposition

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

A priori resolution of the intermediate spectra in the bacteriorhodopsin photocycle : The time evolution of the L spectrum revealed. / Zimányi, L.; Saltiel, Jack; Brown, Leonid S.; Lanyi, Janos K.

In: Journal of Physical Chemistry A, Vol. 110, No. 7, 23.02.2006, p. 2318-2321.

Research output: Contribution to journalArticle

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