A prenyl pyrophosphate synthase gene from the zygomycete fungus, Gilbertella persicaria

Gábor Nagy, Gergely Imre, Árpád Csernetics, Tamás Petkovits, László G. Nagy, A. Szekeres, C. Vágvölgyi, T. Papp

Research output: Contribution to journalArticle

Abstract

In this study, a prenyl pyrophosphate synthase gene was cloned and characterized in the zygomycete fungus Gilbertella persicaria. The coding region of the isolated gene comprises a total of 1328 bp determining a 350 amino acids length putative protein and five introns (60, 65, 43, 59, and 48 bp in length) dispersed in the whole coding region. Based on the analysis of the nucleotide and the corresponding amino acid sequence, the gene encodes a farnezyl pyrophosphate synthase, and it was named as isoA. In a phylogeny inferred from amino acid sequences of fungal prenyl pyrophosphate synthases, the Gilbertella isoA proved to be the most closely related to that of Mucor circinelloides. Carotenoid composition of G. persicaria was also investigated: it produces β-carotene as the main carotenoid, but it also contains small amounts of β-cryptoxanthin, zeaxanthin and astaxanthin.

Original languageEnglish
Pages (from-to)7-12
Number of pages6
JournalActa Biologica Szegediensis
Volume55
Issue number1
Publication statusPublished - 2011

Fingerprint

pyrophosphates
Carotenoids
Fungi
Genes
Amino Acids
fungi
Gilbertella
Amino Acid Sequence
carotenoids
amino acid sequences
Mucor circinelloides
Inteins
Mucor
astaxanthin
genes
zeaxanthin
carotenes
Phylogeny
Introns
introns

Keywords

  • β-carotene
  • Farnezyl pyrophosphate synthase
  • Gilbertella
  • Isoprene biosynthesis
  • Sequence analysis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

A prenyl pyrophosphate synthase gene from the zygomycete fungus, Gilbertella persicaria. / Nagy, Gábor; Imre, Gergely; Csernetics, Árpád; Petkovits, Tamás; Nagy, László G.; Szekeres, A.; Vágvölgyi, C.; Papp, T.

In: Acta Biologica Szegediensis, Vol. 55, No. 1, 2011, p. 7-12.

Research output: Contribution to journalArticle

Nagy, Gábor ; Imre, Gergely ; Csernetics, Árpád ; Petkovits, Tamás ; Nagy, László G. ; Szekeres, A. ; Vágvölgyi, C. ; Papp, T. / A prenyl pyrophosphate synthase gene from the zygomycete fungus, Gilbertella persicaria. In: Acta Biologica Szegediensis. 2011 ; Vol. 55, No. 1. pp. 7-12.
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