Abstract
The contractile activation of the upper (dome) and lower (base) parts of the urinary bladder show some differences. Cellular mechanisms that might be responsible for cholinergic effects blocking non-adrenergic non-cholinergic contractions in the base of the rat urinary bladder were investigated. Smooth muscle cells were thus freshly isolated or cultured both from the dome and the base of the rat urinary bladder and the contribution from cholinergic and purinergic pathways to their Ca2+ homeostasis was examined. The expression of nicotinic acetylcholine (nAChR) and P2X2 purinergic receptors on the cultured cells and on tissue sections was investigated. The ATP-evoked Ca2+ transients in rat smooth muscle cells did not show any desensitization. However, when ATP was administered together with carbamylcholine (CCh), the latter essentially prevented ATP from evoking Ca 2+ transients in smooth muscle cells from the base (suppression to 12 ± 2.5% of control, n = 57; p <0.01), but not from the dome (99 ± 5% of control, n = 52; p > 0.05) of the rat urinary bladder. While atropine was unable to modify (6 ± 3% of control, n = 14; p <0.05), α-bungarotoxin (118 ± 12% of control, n = 20; p > 0.05) blocked the inhibitory effects of CCh. Additionally, α7 subunits of nAChR and P2X2 purinergic receptors were identified using immunocytochemistry, immunohistochemistry, and Western blot in cultured urinary bladder smooth muscle cells, in urinary bladder sections, and in urinary bladder muscle strips, respectively, suggesting that the activation of nAChR modifies the action of ATP.
| Original language | English |
|---|---|
| Pages (from-to) | 421-431 |
| Number of pages | 11 |
| Journal | Journal of Muscle Research and Cell Motility |
| Volume | 32 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Mar 2012 |
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Keywords
- Nicotinic acetylcholine receptors
- Non-adrenergic
- Non-cholinergic contractions
- Purinergic receptors
- Receptor interaction
- Urinary bladder smooth muscle
ASJC Scopus subject areas
- Physiology
- Cell Biology
- Biochemistry
Cite this
A possible role of the cholinergic and purinergic receptor interaction in the regulation of the rat urinary bladder function. / Jenes, Ágnes; Ruzsnavszky, Ferenc; Telek, Andrea; Szigeti, G.; Csernoch, L.
In: Journal of Muscle Research and Cell Motility, Vol. 32, No. 6, 03.2012, p. 421-431.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A possible role of the cholinergic and purinergic receptor interaction in the regulation of the rat urinary bladder function
AU - Jenes, Ágnes
AU - Ruzsnavszky, Ferenc
AU - Telek, Andrea
AU - Szigeti, G.
AU - Csernoch, L.
PY - 2012/3
Y1 - 2012/3
N2 - The contractile activation of the upper (dome) and lower (base) parts of the urinary bladder show some differences. Cellular mechanisms that might be responsible for cholinergic effects blocking non-adrenergic non-cholinergic contractions in the base of the rat urinary bladder were investigated. Smooth muscle cells were thus freshly isolated or cultured both from the dome and the base of the rat urinary bladder and the contribution from cholinergic and purinergic pathways to their Ca2+ homeostasis was examined. The expression of nicotinic acetylcholine (nAChR) and P2X2 purinergic receptors on the cultured cells and on tissue sections was investigated. The ATP-evoked Ca2+ transients in rat smooth muscle cells did not show any desensitization. However, when ATP was administered together with carbamylcholine (CCh), the latter essentially prevented ATP from evoking Ca 2+ transients in smooth muscle cells from the base (suppression to 12 ± 2.5% of control, n = 57; p <0.01), but not from the dome (99 ± 5% of control, n = 52; p > 0.05) of the rat urinary bladder. While atropine was unable to modify (6 ± 3% of control, n = 14; p <0.05), α-bungarotoxin (118 ± 12% of control, n = 20; p > 0.05) blocked the inhibitory effects of CCh. Additionally, α7 subunits of nAChR and P2X2 purinergic receptors were identified using immunocytochemistry, immunohistochemistry, and Western blot in cultured urinary bladder smooth muscle cells, in urinary bladder sections, and in urinary bladder muscle strips, respectively, suggesting that the activation of nAChR modifies the action of ATP.
AB - The contractile activation of the upper (dome) and lower (base) parts of the urinary bladder show some differences. Cellular mechanisms that might be responsible for cholinergic effects blocking non-adrenergic non-cholinergic contractions in the base of the rat urinary bladder were investigated. Smooth muscle cells were thus freshly isolated or cultured both from the dome and the base of the rat urinary bladder and the contribution from cholinergic and purinergic pathways to their Ca2+ homeostasis was examined. The expression of nicotinic acetylcholine (nAChR) and P2X2 purinergic receptors on the cultured cells and on tissue sections was investigated. The ATP-evoked Ca2+ transients in rat smooth muscle cells did not show any desensitization. However, when ATP was administered together with carbamylcholine (CCh), the latter essentially prevented ATP from evoking Ca 2+ transients in smooth muscle cells from the base (suppression to 12 ± 2.5% of control, n = 57; p <0.01), but not from the dome (99 ± 5% of control, n = 52; p > 0.05) of the rat urinary bladder. While atropine was unable to modify (6 ± 3% of control, n = 14; p <0.05), α-bungarotoxin (118 ± 12% of control, n = 20; p > 0.05) blocked the inhibitory effects of CCh. Additionally, α7 subunits of nAChR and P2X2 purinergic receptors were identified using immunocytochemistry, immunohistochemistry, and Western blot in cultured urinary bladder smooth muscle cells, in urinary bladder sections, and in urinary bladder muscle strips, respectively, suggesting that the activation of nAChR modifies the action of ATP.
KW - Nicotinic acetylcholine receptors
KW - Non-adrenergic
KW - Non-cholinergic contractions
KW - Purinergic receptors
KW - Receptor interaction
KW - Urinary bladder smooth muscle
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UR - http://www.scopus.com/inward/citedby.url?scp=84858446575&partnerID=8YFLogxK
U2 - 10.1007/s10974-012-9285-x
DO - 10.1007/s10974-012-9285-x
M3 - Article
C2 - 22370867
AN - SCOPUS:84858446575
VL - 32
SP - 421
EP - 431
JO - Journal of Muscle Research and Cell Motility
JF - Journal of Muscle Research and Cell Motility
SN - 0142-4319
IS - 6
ER -