A possible role of the cholinergic and purinergic receptor interaction in the regulation of the rat urinary bladder function

Ágnes Jenes, Ferenc Ruzsnavszky, Andrea Telek, G. Szigeti, L. Csernoch

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The contractile activation of the upper (dome) and lower (base) parts of the urinary bladder show some differences. Cellular mechanisms that might be responsible for cholinergic effects blocking non-adrenergic non-cholinergic contractions in the base of the rat urinary bladder were investigated. Smooth muscle cells were thus freshly isolated or cultured both from the dome and the base of the rat urinary bladder and the contribution from cholinergic and purinergic pathways to their Ca2+ homeostasis was examined. The expression of nicotinic acetylcholine (nAChR) and P2X2 purinergic receptors on the cultured cells and on tissue sections was investigated. The ATP-evoked Ca2+ transients in rat smooth muscle cells did not show any desensitization. However, when ATP was administered together with carbamylcholine (CCh), the latter essentially prevented ATP from evoking Ca 2+ transients in smooth muscle cells from the base (suppression to 12 ± 2.5% of control, n = 57; p <0.01), but not from the dome (99 ± 5% of control, n = 52; p > 0.05) of the rat urinary bladder. While atropine was unable to modify (6 ± 3% of control, n = 14; p <0.05), α-bungarotoxin (118 ± 12% of control, n = 20; p > 0.05) blocked the inhibitory effects of CCh. Additionally, α7 subunits of nAChR and P2X2 purinergic receptors were identified using immunocytochemistry, immunohistochemistry, and Western blot in cultured urinary bladder smooth muscle cells, in urinary bladder sections, and in urinary bladder muscle strips, respectively, suggesting that the activation of nAChR modifies the action of ATP.

Original languageEnglish
Pages (from-to)421-431
Number of pages11
JournalJournal of Muscle Research and Cell Motility
Volume32
Issue number6
DOIs
Publication statusPublished - Mar 2012

Fingerprint

Purinergic Receptors
Cholinergic Receptors
Muscle
Rats
Urinary Bladder
Cells
Purinergic P2X2 Receptors
Adenosine Triphosphate
Smooth Muscle Myocytes
Domes
Carbachol
Cholinergic Agents
Chemical activation
Immunohistochemistry
Atropine
Acetylcholine
Tissue
Cultured Cells
Homeostasis
Western Blotting

Keywords

  • Nicotinic acetylcholine receptors
  • Non-adrenergic
  • Non-cholinergic contractions
  • Purinergic receptors
  • Receptor interaction
  • Urinary bladder smooth muscle

ASJC Scopus subject areas

  • Physiology
  • Cell Biology
  • Biochemistry

Cite this

A possible role of the cholinergic and purinergic receptor interaction in the regulation of the rat urinary bladder function. / Jenes, Ágnes; Ruzsnavszky, Ferenc; Telek, Andrea; Szigeti, G.; Csernoch, L.

In: Journal of Muscle Research and Cell Motility, Vol. 32, No. 6, 03.2012, p. 421-431.

Research output: Contribution to journalArticle

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