A polymerizable GFP variant

Ágnes Klein, Balaázs ToÁth, Hajnalka Jankovics, Adeál MuskotaÁl, F. Vonderviszt

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Flagellin has the ability to polymerize into long filaments under appropriate conditions. Our work aims at the construction of flagellin-based fusion proteins which possess polymerization ability and preserve the functional properties of the fusion partner as well. The hypervariable D3 domain of Salmonella flagellin, containing residues 190-283, is a good target for genetic engineering studies since it can be extensively modified or removed without disturbing the self-assembling ability. In this work a fusion construct of flagellin and the superfolder mutant of the green fluorescent protein were created by replacing D3 with superfolder green fluorescent protein (GFP). The obtained GFP variant was capable of forming stable, highly fluorescent filamentous assemblies. Our results imply that other proteins (enzymes, binding proteins, etc.) can also be furnished by polymerization ability in a similar way. This approach paves the way for the construction of multifunctional tubular nanostructures.

Original languageEnglish
Pages (from-to)153-157
Number of pages5
JournalProtein Engineering, Design and Selection
Volume25
Issue number4
DOIs
Publication statusPublished - Apr 2012

Fingerprint

Flagellin
Green Fluorescent Proteins
Proteins
Fusion reactions
Polymerization
Genetic engineering
Salmonella
Genetic Engineering
Nanostructures
Protein Binding
Carrier Proteins
Enzymes

Keywords

  • flagellin
  • GFP
  • polymerization
  • self-assembly
  • tubular nanostructure

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Molecular Biology

Cite this

A polymerizable GFP variant. / Klein, Ágnes; ToÁth, Balaázs; Jankovics, Hajnalka; MuskotaÁl, Adeál; Vonderviszt, F.

In: Protein Engineering, Design and Selection, Vol. 25, No. 4, 04.2012, p. 153-157.

Research output: Contribution to journalArticle

Klein, Á, ToÁth, B, Jankovics, H, MuskotaÁl, A & Vonderviszt, F 2012, 'A polymerizable GFP variant', Protein Engineering, Design and Selection, vol. 25, no. 4, pp. 153-157. https://doi.org/10.1093/protein/gzs003
Klein, Ágnes ; ToÁth, Balaázs ; Jankovics, Hajnalka ; MuskotaÁl, Adeál ; Vonderviszt, F. / A polymerizable GFP variant. In: Protein Engineering, Design and Selection. 2012 ; Vol. 25, No. 4. pp. 153-157.
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