A physical method for separating Saccharomyces cerevisiae cells according to their ploidy.

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Abstract

A centrifugation technique, using genetically marked Saccharomyces cerevisiae strains, has been developed to separate Saccharomyces cerevisiae cells of different ploidy levels from exponential phase cultures. The method involves the conversion of yeast cells to protoplasts, the separation of the protoplasts on an osmotically stabilized Nycodenz gradient, and their regeneration. This type of selection may be of importance where selectable markers are not available.

Original languageEnglish
Pages (from-to)1102-1104
Number of pages3
JournalCanadian journal of microbiology
Volume34
Issue number9
DOIs
Publication statusPublished - Sep 1988

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ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics

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